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Literature summary for 2.7.1.36 extracted from

  • Suarez, D.; Garcia-Peregrin, E.
    Properties and partial purification of mevalonate kinase from Agave americana (1977), Phytochemistry, 16, 661-665.
No PubMed abstract available
Search for more literature for 2.7.1.36

General Stability

General Stability Organism
sensitive to dialysis Agave americana

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ inhibition at high concentrations Agave americana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 mevalonate pH 7.9, 37°C Agave americana
0.14
-
 ATP pH 7.9, 37°C Agave americana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 6-8 mM Agave americana
Mn2+ maximal acitvation at 2 mM, inhibition above Agave americana

Organism

Organism UniProt Comment Textmining
Agave americana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-100, DEAE-Sephadex Agave americana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Agave americana
-
leaf
-
 Agave americana
-
scape
-
 Agave americana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.58 dpm/mg Agave americana

Storage Stability

Storage Stability Organism
5°C, stable for several months in buffer containing dithiothreitol Agave americana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + mevalonate ATP is the most effective phosphate donor Agave americana ADP + phosphomevalonate
-
 ?
GTP + mevalonate
-
 Agave americana GDP + phosphomevalonate
-
 ?
ITP + mevalonate
-
 Agave americana IDP + phosphomevalonate
-
 ?
UTP + mevalonate
-
 Agave americana UDP + phosphomevalonate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Agave americana