Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | hyperbolic activation curve, 13% activation at 25 mM, 87% activation at 500 mM | Sus scrofa | |
ATP4- | most effective activator, maximal activation at 8 mM | Sus scrofa | |
phosphate | 50% activation at 100 mM | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | inhibition above 2.5 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
ATP | pH 6.5, 30°C, at 1 mM Mg2+ | Sus scrofa | |
0.68 | - |
ATP | ATP in form of MgATP2- | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Ba2+ | can partially replace Mg2+ in activation | Sus scrofa | |
Ba2+ | at 5 mM 10% of activity with Mg2+ | Sus scrofa | |
Ca2+ | can partially replace Mn2+ in activation | Rattus norvegicus | |
Ca2+ | can partially replace Mn2+ in activation | Sus scrofa | |
Ca2+ | 71% of activity with Mg2+ | Sus scrofa | |
Mg2+ | presence of free ATP4- results in a biphasic Lineweaver-Burk plot with 2 apparent Km-values for MgATP2-: 0.14 mM and 0.006 mM | Rattus norvegicus | |
Mg2+ | presence of free ATP4- results in a biphasic Lineweaver-Burk plot with 2 apparent Km-values for MgATP2-: 0.14 mM and 0.006 mM | Sus scrofa | |
Mn2+ | can partially replace Mg2+ in activation, maximal activation at 2.5 mM, inhibition above | Sus scrofa | |
Mn2+ | most active at low concentrations | Sus scrofa | |
Sr2+ | can partially replace Mg2+ in activation, 8% of activity with Mg2+ at 5 mM | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Sus scrofa |
104000 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Blue-Sepharose, DEAE-Trisacryl, ATP-agarose, Sephadex G-150 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay procedure | Sus scrofa |
30.4 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Sus scrofa | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + mevalonate | ATP is the most effective phosphate donor | Rattus norvegicus | ADP + phosphomevalonate | - |
? | |
ATP + mevalonate | ATP is the most effective phosphate donor | Sus scrofa | ADP + phosphomevalonate | - |
ir | |
CTP + mevalonate | 18% of activity with ATP | Sus scrofa | CDP + phosphomevalonate | - |
? | |
dATP + mevalonate | 67% of the activity with ATP | Sus scrofa | dADP + phosphomevalonate | - |
? | |
dCTP + mevalonate | 17% of the activity with ATP | Sus scrofa | dCDP + phosphomevalonate | - |
? | |
dGTP + mevalonate | 17% of the activity with ATP | Sus scrofa | dGDP + phosphomevalonate | - |
? | |
GTP + mevalonate | 18% of the activity with ATP | Sus scrofa | GDP + phosphomevalonate | - |
? | |
ITP + mevalonate | 88% of activity with ATP | Sus scrofa | IDP + phosphomevalonate | - |
? | |
TTP + mevalonate | 15% of the activity with ATP | Sus scrofa | TDP + phosphomevalonate | - |
? | |
UTP + mevalonate | 61% of the activity with ATP | Sus scrofa | UDP + phosphomevalonate | - |
? | |
XTP + mevalonate | 27% of the activity with ATP | Sus scrofa | XDP + phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rattus norvegicus |
dimer | 2 * 52000, SDS-PAGE | Sus scrofa |