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Literature summary for 2.7.1.36 extracted from

  • Soong Lee, C.; O'Sullivan, W.J.
    An improved purification procedure, an alternative assay and activation of mevalonate kinase by ATP (1983), Biochim. Biophys. Acta, 747, 215-224.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
arsenate hyperbolic activation curve, 13% activation at 25 mM, 87% activation at 500 mM Sus scrofa
ATP4- most effective activator, maximal activation at 8 mM Sus scrofa
phosphate 50% activation at 100 mM Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ inhibition above 2.5 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
ATP pH 6.5, 30°C, at 1 mM Mg2+ Sus scrofa
0.68
-
ATP ATP in form of MgATP2- Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ can partially replace Mg2+ in activation Rattus norvegicus
Ba2+ can partially replace Mg2+ in activation Sus scrofa
Ba2+ at 5 mM 10% of activity with Mg2+ Sus scrofa
Ca2+ can partially replace Mn2+ in activation Rattus norvegicus
Ca2+ can partially replace Mn2+ in activation Sus scrofa
Ca2+ 71% of activity with Mg2+ Sus scrofa
Mg2+ presence of free ATP4- results in a biphasic Lineweaver-Burk plot with 2 apparent Km-values for MgATP2-: 0.14 mM and 0.006 mM Rattus norvegicus
Mg2+ presence of free ATP4- results in a biphasic Lineweaver-Burk plot with 2 apparent Km-values for MgATP2-: 0.14 mM and 0.006 mM Sus scrofa
Mn2+ can partially replace Mg2+ in activation, maximal activation at 2.5 mM, inhibition above Sus scrofa
Mn2+ most active at low concentrations Sus scrofa
Sr2+ can partially replace Mg2+ in activation, 8% of activity with Mg2+ at 5 mM Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, SDS-PAGE Sus scrofa
104000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Blue-Sepharose, DEAE-Trisacryl, ATP-agarose, Sephadex G-150 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay procedure Sus scrofa
30.4
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
Sus scrofa ADP + (R)-5-phosphomevalonate
-
?
ATP + mevalonate ATP is the most effective phosphate donor Rattus norvegicus ADP + phosphomevalonate
-
?
ATP + mevalonate ATP is the most effective phosphate donor Sus scrofa ADP + phosphomevalonate
-
ir
CTP + mevalonate 18% of activity with ATP Sus scrofa CDP + phosphomevalonate
-
?
dATP + mevalonate 67% of the activity with ATP Sus scrofa dADP + phosphomevalonate
-
?
dCTP + mevalonate 17% of the activity with ATP Sus scrofa dCDP + phosphomevalonate
-
?
dGTP + mevalonate 17% of the activity with ATP Sus scrofa dGDP + phosphomevalonate
-
?
GTP + mevalonate 18% of the activity with ATP Sus scrofa GDP + phosphomevalonate
-
?
ITP + mevalonate 88% of activity with ATP Sus scrofa IDP + phosphomevalonate
-
?
TTP + mevalonate 15% of the activity with ATP Sus scrofa TDP + phosphomevalonate
-
?
UTP + mevalonate 61% of the activity with ATP Sus scrofa UDP + phosphomevalonate
-
?
XTP + mevalonate 27% of the activity with ATP Sus scrofa XDP + phosphomevalonate
-
?

Subunits

Subunits Comment Organism
dimer
-
Rattus norvegicus
dimer 2 * 52000, SDS-PAGE Sus scrofa