Cloned (Comment) | Organism |
---|---|
gene coaX, functional recombinant expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of acetyl-CoA carboxylase (acc) obtained from Corynebacterium glutamicum in Escherichia coli, it causes accumulation of 2.2fold more fatty acids in Escherichia coli that in the wild-type. The addition of gene coaX encoding patothenate kinase from Pseudomonas putida or fatty acid synthase (fasA) from Corynebacterium glutamicum results in a 3.1 and 3.6fold increased fatty acid synthesis in Escherichia coli cells, which express acc and coaA, or acc and fasA, respectively. The transformants, simultaneously possessing all three genes, produce 5.6fold more fatty acids. The strain possessing acc, coaA, and fasA stores 691 mg/l of fatty acids, primarily as phospholipids, inside the inner membrane after 72-h cultivation. In addition, 19% of the total CoA pool is occupied by malonyl-CoA | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | Pseudomonas putida | - |
ADP + (R)-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | V5XWU6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Pseudomonas putida | ADP + (R)-4'-phosphopantothenate | - |
? |