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Literature summary for 2.7.1.33 extracted from

  • Takagi, M.; Tamaki, H.; Miyamoto, Y.; Leonardi, R.; Hanada, S.; Jackowski, S.; Chohnan, S.
    Pantothenate kinase from the thermoacidophilic archaeon Picrophilus torridus (2010), J. Bacteriol., 192, 233-241.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Picrophilus torridus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.099
-
ATP
-
Picrophilus torridus
0.621
-
pantothenate
-
Picrophilus torridus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity with 1 mM (plus 2.5 mM ATP): 252 nmol/min/mg Picrophilus torridus
Mn2+ addition (1 mM) instead of Mg2+ accelerates activity 1.75fold Picrophilus torridus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000 Da, SDS-PAGE Picrophilus torridus

Organism

Organism UniProt Comment Textmining
Picrophilus torridus Q6L2I5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Picrophilus torridus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
purified recombinant protein Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pantothenate + ATP relative activity with GTP: 81%, CTP: 68%, and UTP: 91.8% Picrophilus torridus phosphopantothenate + ADP
-
?

Subunits

Subunits Comment Organism
? x * 33000 Da, SDS-PAGE Picrophilus torridus

Synonyms

Synonyms Comment Organism
pantothenate kinase
-
Picrophilus torridus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Picrophilus torridus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 more than 80% of the maximum activity Picrophilus torridus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.718
-
ATP
-
Picrophilus torridus
0.818
-
pantothenate
-
Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Picrophilus torridus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 more than 70% of the maximum activity Picrophilus torridus