Cloned (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
- |
Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 28% (w/v) PEG 2000 mM and 0.1M Bis-Tris (pH 6.5), at 18°C | Pseudomonas aeruginosa |
sitting drop vapour diffusion method using 28% (w/v) PEG 2 M and 0.1 M Bis-Tris (pH 6.5), at 18°C | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D101A | reduced enzymatic activity | Pseudomonas aeruginosa |
D121A | reduced enzymatic activity | Pseudomonas aeruginosa |
D6A | reduced enzymatic activity | Staphylococcus aureus |
E70A | reduced enzymatic activity | Staphylococcus aureus |
H156A | slightly increased enzymatic activity | Pseudomonas aeruginosa |
K13A | reduced enzymatic activity | Staphylococcus aureus |
K13A | reduced enzymatic activity | Pseudomonas aeruginosa |
L11A | reduced enzymatic activity | Staphylococcus aureus |
L263P | reduced enzymatic activity | Staphylococcus aureus |
N9G | strongly reduced enzymatic activity | Pseudomonas aeruginosa |
T10A | reduced enzymatic activity | Staphylococcus aureus |
T157A | reduced enzymatic activity | Pseudomonas aeruginosa |
Y137A | reduced enzymatic activity | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 0.06-0.12 mM | Staphylococcus aureus | |
K+ | 0.06-0.12 mM | Pseudomonas aeruginosa | |
Mg2+ | required | Staphylococcus aureus | |
Mg2+ | required | Pseudomonas aeruginosa | |
NH4+ | induces a 5fold higher activation than either K+ or Rb+ | Staphylococcus aureus | |
NH4+ | induces a 5fold higher activation than either K+ or Rb+ | Pseudomonas aeruginosa | |
Rb+ | - |
Staphylococcus aureus | |
Rb+ | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HWC1 | - |
- |
Staphylococcus aureus | Q6G7I0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Staphylococcus aureus | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Pseudomonas aeruginosa | ADP + (R)-4'-phosphopantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Staphylococcus aureus |
dimer | x-ray crystallography | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
CoaA | - |
Staphylococcus aureus |
CoaA | - |
Pseudomonas aeruginosa |
type II pantothenate kinase | - |
Staphylococcus aureus |
type III pantothenate kinase | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus | |
ATP | - |
Pseudomonas aeruginosa |