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Literature summary for 2.7.1.33 extracted from

  • Leonardi, R.; Rock, C.O.; Jackowski, S.; Zhang, Y.M.
    Activation of human mitochondrial pantothenate kinase 2 by palmitoylcarnitine (2007), Proc. Natl. Acad. Sci. USA, 104, 1494-1499.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.33

Activating Compound

Activating Compound Comment Organism Structure
additional information carnitine and octanoylcarnitine do not have any effect on the PanK2 activity Homo sapiens
palmitoylcarnitine activates Homo sapiens
palmitoylcarnitine the enzymatic activity of PanK2 is stimulated to the highest level by 0.008 mM palmitoylcarnitine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA competitive inhibitor of purified PanK2 with respect to ATP; IC50: 60 nM, competitive inhibitor with respect to ATP Homo sapiens
palmitoyl-CoA strong inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion PanK2 is located in the mitochondria to sense the levels of palmitoylcarnitine and up-regulate CoA biosynthesis in response to an increased mitochondrial demand for the cofactor to support beta-oxidation Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q9BZ23
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged PanK2 Homo sapiens
HiTrap chelating HP column chromatography and Superdex 200 column gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002421
-
 0.045 mM pantothenate, 0.25 mM ATP (pH 7.0), 10 mM MgCl2, 0.1 M Tris/HCl (pH 7.5), at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Homo sapiens ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + D-pantothenate
-
 Homo sapiens ADP + 4'-phosphopantothenate
-
 ?

Synonyms

Synonyms Comment Organism
PanK2
-
 Homo sapiens
pantothenate kinase 2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00006
-
 IC50: 60 nM, competitive inhibitor with respect to ATP Homo sapiens acetyl-CoA