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Literature summary for 2.7.1.33 extracted from

  • Leonardi, R.; Chohnan, S.; Zhang, Y.M.; Virga, K.G.; Lee, R.E.; Rock, C.O.; Jackowski, S.
    A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A (2005), J. Biol. Chem., 280, 3314-3322.
    View publication on PubMed
Search for more literature for 2.7.1.33

Activating Compound

Activating Compound Comment Organism Structure
additional information no feedback regulation by CoA or acetyl-CoA Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
gene coaA, expression of His6-tagged in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
additional information no feedback regulation by CoA or acetyl-CoA Staphylococcus aureus
N-heptylpantothenamide IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite Staphylococcus aureus
N-pentylpantothenamide IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 N-pentylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
0.008
-
 N-heptylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
0.023
-
 (R)-pantothenate pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
0.034
-
 ATP pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000, recombinant enzyme, SDS-PAGE Staphylococcus aureus
59000
-
 recombinant enzyme, gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantothenate Staphylococcus aureus first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate Staphylococcus aureus RN4220 first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + N-heptylpantothenamide Staphylococcus aureus substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview ADP + N-heptylpantothenamide 4-phosphate
-
 ?
ATP + N-heptylpantothenamide Staphylococcus aureus RN4220 substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview ADP + N-heptylpantothenamide 4-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
 gene coaA
-
Staphylococcus aureus RN4220
-
 gene coaA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate first step in coenzyme A biosynthesis Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate
-
 Staphylococcus aureus RN4220 ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate first step in coenzyme A biosynthesis Staphylococcus aureus RN4220 ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + N-heptylpantothenamide
-
 Staphylococcus aureus ADP + N-heptylpantothenamide 4-phosphate
-
 ?
ATP + N-heptylpantothenamide substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview Staphylococcus aureus ADP + N-heptylpantothenamide 4-phosphate
-
 ?
ATP + N-heptylpantothenamide
-
 Staphylococcus aureus RN4220 ADP + N-heptylpantothenamide 4-phosphate
-
 ?
ATP + N-heptylpantothenamide substrate is converted to the inactive butyldethia-CoA analogue in the further downstream pathway leading to grwoth inhibition of the organism, overview Staphylococcus aureus RN4220 ADP + N-heptylpantothenamide 4-phosphate
-
 ?
ATP + N-pentylpantothenamide
-
 Staphylococcus aureus ADP + N-pentylpantothenamide 4-phosphate
-
 ?
ATP + N-pentylpantothenamide
-
 Staphylococcus aureus RN4220 ADP + N-pentylpantothenamide 4-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29000, recombinant enzyme, SDS-PAGE Staphylococcus aureus

Synonyms

Synonyms Comment Organism
CoaA
-
 Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 N-pentylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
0.425
-
 N-heptylpantothenamide pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
1.6
-
 ATP pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus
1.7
-
 (R)-pantothenate pH 7.5, 37°C, recombinant enzyme Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Staphylococcus aureus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0035
-
 IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus Staphylococcus aureus N-pentylpantothenamide
0.0048
-
 IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite Staphylococcus aureus N-heptylpantothenamide