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Literature summary for 2.7.1.32 extracted from

  • Gee, P.; Kent, C.
    Multiple isoforms of choline kinase from Caenorhabditis elegans: cloning, expression, purification, and characterization (2003), Biochim. Biophys. Acta, 1648, 33-42.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
7 different genes, cloned 5 Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
ATP isozyme CKB-2, pH 10.0, 37°C Caenorhabditis elegans
1.6
-
choline isozyme CKA-2, pH 10.0, 37°C Caenorhabditis elegans
2.4
-
ATP isozyme CKA-2, pH 10.0, 37°C Caenorhabditis elegans
13
-
choline isozyme CKB-2, pH 10.0, 37°C Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Caenorhabditis elegans
Mn2+ poor substitute for Mg2+ Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40600
-
isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence Caenorhabditis elegans
42000
-
isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence Caenorhabditis elegans
48600
-
isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence Caenorhabditis elegans
49000
-
isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoforms CKA-2, CKB-2 Caenorhabditis elegans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
isozyme CKB-2, pH 10.0, 37°C Caenorhabditis elegans
43
-
isozyme CKA-2, pH 10.0, 37°C Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
Caenorhabditis elegans ADP + O-phosphocholine
-
?
ATP + ethanolamine poor substrate compared to choline Caenorhabditis elegans ADP + ethanolamine phosphate
-
?

Subunits

Subunits Comment Organism
? isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence Caenorhabditis elegans
dimer isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
isoform CKA-2 Caenorhabditis elegans