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Literature summary for 2.7.1.31 extracted from

  • Kleczkowski, L.A.; Randall, D.D.
    Purification and partial characterization of spinach leaf glycerate kinase (1983), FEBS Lett., 158, 313-316.
No PubMed abstract available
Search for more literature for 2.7.1.31

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Spinacia oleracea
0.285
-
 D-glycerate 25°C, pH 7.8 Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 1 * 39500, SDS-PAGE Spinacia oleracea
40000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Spinacia oleracea

Storage Stability

Storage Stability Organism
purified enzyme totally unstable after overnight storage in a freezer unless bovine serum albumin, 3 mg/ml, is added Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate specific for D-isomer Spinacia oleracea ADP + 3-phospho-(R)-glycerate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39500, SDS-PAGE Spinacia oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
 Spinacia oleracea