BRENDA - Enzyme Database
show all sequences of 2.7.1.31

Partial purification and characterization of glycerate kinase from immature pods of Brassica campestris

Saharan, M.M.R.; Singh, R.; Plant Physiol. Biochem. 31, 559-566 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
diphosphate
-
Brassica rapa subsp. oleifera
fructose 6-phosphate
weak
Brassica rapa subsp. oleifera
glucose 1-phosphate
weak
Brassica rapa subsp. oleifera
glucose 6-phosphate
weak
Brassica rapa subsp. oleifera
glycolate
-
Brassica rapa subsp. oleifera
pyruvate
noncompetitive to glycerate, competitive to ATP
Brassica rapa subsp. oleifera
ribose 5-phosphate
-
Brassica rapa subsp. oleifera
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
ATP
-
Brassica rapa subsp. oleifera
0.17
-
D-glycerate
-
Brassica rapa subsp. oleifera
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement for Mg2+
Brassica rapa subsp. oleifera
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
-
Brassica rapa subsp. oleifera
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica rapa subsp. oleifera
-
-
-
Purification (Commentary)
Commentary
Organism
-
Brassica rapa subsp. oleifera
Reaction
Reaction
Commentary
Organism
ATP + D-glycerate = ADP + 3-phospho-D-glycerate
sequential binding of substrates
Brassica rapa subsp. oleifera
Source Tissue
Source Tissue
Commentary
Organism
Textmining
pod
immature
Brassica rapa subsp. oleifera
-
Storage Stability
Storage Stability
Organism
4C, quite stable
Brassica rapa subsp. oleifera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-glycerate
-
641332
Brassica rapa subsp. oleifera
ADP + 3-phospho-(R)-glycerate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000
Brassica rapa subsp. oleifera
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Brassica rapa subsp. oleifera
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diphosphate
-
Brassica rapa subsp. oleifera
fructose 6-phosphate
weak
Brassica rapa subsp. oleifera
glucose 1-phosphate
weak
Brassica rapa subsp. oleifera
glucose 6-phosphate
weak
Brassica rapa subsp. oleifera
glycolate
-
Brassica rapa subsp. oleifera
pyruvate
noncompetitive to glycerate, competitive to ATP
Brassica rapa subsp. oleifera
ribose 5-phosphate
-
Brassica rapa subsp. oleifera
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
ATP
-
Brassica rapa subsp. oleifera
0.17
-
D-glycerate
-
Brassica rapa subsp. oleifera
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement for Mg2+
Brassica rapa subsp. oleifera
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
-
Brassica rapa subsp. oleifera
Purification (Commentary) (protein specific)
Commentary
Organism
-
Brassica rapa subsp. oleifera
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
pod
immature
Brassica rapa subsp. oleifera
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, quite stable
Brassica rapa subsp. oleifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-glycerate
-
641332
Brassica rapa subsp. oleifera
ADP + 3-phospho-(R)-glycerate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000
Brassica rapa subsp. oleifera
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Brassica rapa subsp. oleifera
Other publictions for EC 2.7.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739413
Zelcbuch
An in vivo metabolic approach ...
Arabidopsis thaliana, no activity in Escherichia coli
PLoS ONE
10
e0122957
2015
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1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
723461
Bartsch
An autoinhibitory domain confe ...
Zea mays
Plant Physiol.
153
832-840
2010
2
-
1
-
-
-
-
4
-
-
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-
5
-
-
1
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1
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1
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2
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1
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4
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1
-
1
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1
-
-
-
-
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-
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-
1
1
1
1
-
-
686769
Bartsch
Only plant-type (GLYK) glycera ...
Arabidopsis thaliana, Nostoc sp. PCC 7120, Oryza sativa, Saccharomyces cerevisiae
FEBS Lett.
582
3025-3028
2008
-
-
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8
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4
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4
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8
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4
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673281
Guo
Isolation and characterization ...
Homo sapiens, Mus musculus
DNA Seq.
17
1-7
2006
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2
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3
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4
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25
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2
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3
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25
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660135
Boldt
D-Glycerate 3-kinase, the last ...
Arabidopsis thaliana, Saccharomyces cerevisiae
Plant Cell
17
2413-2420
2005
-
-
2
-
1
-
1
-
-
-
1
2
-
10
-
-
2
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-
1
3
-
6
1
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2
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1
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1
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1
2
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2
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1
3
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6
1
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-
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-
-
-
-
-
-
-
641332
Saharan
-
Partial purification and chara ...
Brassica rapa subsp. oleifera
Plant Physiol. Biochem.
31
559-566
1993
-
-
-
-
-
-
7
2
-
1
1
-
-
1
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-
1
1
-
1
-
1
1
1
-
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-
1
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7
-
2
-
1
1
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-
-
1
-
1
-
1
1
1
-
-
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-
1
-
-
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-
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-
641342
Kleczkowski
-
Substrate stereospecificity of ...
Spinacia oleracea, Zea mays
Phytochemistry
27
1269-1273
1988
-
-
-
-
-
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2
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2
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2
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2
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2
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2
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2
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2
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2
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641343
Kleczkowski
Purification and characterizat ...
Zea mays
Planta
173
221-229
1988
-
-
-
-
-
1
3
3
-
-
2
-
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3
-
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1
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1
1
1
1
1
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1
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1
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3
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3
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2
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1
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1
1
1
1
1
-
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-
1
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-
-
641333
Chaguturu
-
Glycerate kinase from spinach ...
Spinacia oleracea
Physiol. Plant.
63
19-24
1985
-
-
-
-
-
-
-
-
1
1
-
1
-
1
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1
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1
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2
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1
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1
1
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1
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1
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1
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2
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1
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641341
Kleczkowski
The substrate specificity, kin ...
Spinacia oleracea
Arch. Biochem. Biophys.
236
185-194
1985
-
-
-
-
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-
2
2
-
2
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-
2
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-
1
1
-
1
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4
-
1
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1
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2
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2
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2
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1
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1
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4
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1
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1
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641337
Kleczkowski
-
Purification and partial chara ...
Spinacia oleracea
FEBS Lett.
158
313-316
1983
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-
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-
2
-
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2
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1
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1
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1
1
1
1
1
1
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1
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2
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2
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1
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1
1
1
1
1
1
-
-
-
1
-
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-
-
641340
Schmitt
Glycerate kinase from leaves o ...
Avena sativa, Hordeum vulgare, Pisum sativum, Secale cereale
Arch. Biochem. Biophys.
224
332-341
1983
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4
20
4
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13
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4
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4
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12
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4
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4
20
4
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4
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4
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12
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4
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641331
Doughty
D-glycerate 3-kinase from Esch ...
Escherichia coli
Methods Enzymol.
42
124-127
1975
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1
1
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5
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2
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1
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2
2
1
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1
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2
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1
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1
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5
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1
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2
2
1
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1
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2
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641287
Thorner
-
Glycerol and glycerate kinases ...
Escherichia coli
The Enzymes,3rd ed. (Boyer,P. D. ,ed. )
8
487-508
1973
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-
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1
1
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3
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1
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1
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2
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1
1
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1
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1
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3
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1
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2
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1
1
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641330
Doughty
Purification and properties of ...
Escherichia coli
J. Biol. Chem.
241
568-572
1966
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1
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4
1
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5
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2
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1
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2
2
1
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1
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1
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1
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5
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1
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2
1
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1
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2
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641329
Ichihara
Studies on the purification an ...
Bos taurus, Equus caballus, Rattus norvegicus
J. Biol. Chem.
225
949-958
1957
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9
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15
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3
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3
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3
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3
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3
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3
3
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9
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15
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3
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3
3
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