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Literature summary for 2.7.1.31 extracted from
- Partial purification and characterization of glycerate kinase from immature pods of Brassica campestris (1993), Plant Physiol. Biochem., 31, 559-566.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Brassica rapa subsp. oleifera |  |
| fructose 6-phosphate | weak | Brassica rapa subsp. oleifera | |
| glucose 1-phosphate | weak | Brassica rapa subsp. oleifera | |
| glucose 6-phosphate | weak | Brassica rapa subsp. oleifera | |
| glycolate | - |
Brassica rapa subsp. oleifera | |
| pyruvate | noncompetitive to glycerate, competitive to ATP | Brassica rapa subsp. oleifera | |
| ribose 5-phosphate | - |
Brassica rapa subsp. oleifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ATP | - |
Brassica rapa subsp. oleifera | |
| 0.17 | - |
D-glycerate | - |
Brassica rapa subsp. oleifera | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for Mg2+ | Brassica rapa subsp. oleifera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
- |
Brassica rapa subsp. oleifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica rapa subsp. oleifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica rapa subsp. oleifera |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-glycerate = ADP + 3-phospho-D-glycerate | sequential binding of substrates | Brassica rapa subsp. oleifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pod | immature | Brassica rapa subsp. oleifera | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, quite stable | Brassica rapa subsp. oleifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-glycerate | - |
Brassica rapa subsp. oleifera | ADP + 3-phospho-(R)-glycerate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000 | Brassica rapa subsp. oleifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Brassica rapa subsp. oleifera |
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