Literature summary for 2.7.1.30 extracted from

Zhou, J.; Qu, G.; Zhang, G.; Wu, Z.; Liu, J.; Yang, D.; Li, J.; Chang, M.; Zeng, H.; Hu, J.; Fang, T.; Song, Y.; Bai, C.
Glycerol kinase 5 confers gefitinib resistance through SREBP1/SCD1 signaling pathway (2019), J. Exp. Clin. Cancer Res., 38, 96 .

Search for more literature for 2.7.1.30

Application

Application	Comment	Organism
drug development	glycerol kinase 5 (GK5) is a potential therapeutic target for treatment of NSCLC with resistance to EGFR tyrosine kinase inhibitors	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
transfection of the enzyme in to adenocarcinma cell lines PC-9 and H1975 using a lentiviral vector (Lv105), quantitative RT-PCR enzyme expression analysis	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	silencing using short hairpin RNA (shRNA) vectors against the GK5 genes shGK5-1 and shGK5-2. shGK5-1 and -2 significantly inhibit GK5 expressions. Morphological examination shows that GK5 knockdown reduces the number of PC9R cells transfected with shGK5 compared to those transfected with negative control shRNA for 24 h and then treated with 0.001 mM gefitinib for 72 h. The CCK8 assays reveals that transfection of PC9R cells with either shGK5-1 or -2 enhances gefitinib-induced apoptosis. GK5 knockdown induces PC9R cell apoptosis and cell cycle arrest in the presence of gefitinib	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + glycerol	Homo sapiens	-	ADP + sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6ZS86	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
lung	-	Homo sapiens	-
lung adenocarcinoma cell	-	Homo sapiens	-
NCI-H1975 cell	-	Homo sapiens	-
non-small cell lung cancer cell	-	Homo sapiens	-
PC-9 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + glycerol	-	Homo sapiens	ADP + sn-glycerol 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
GK5	-	Homo sapiens
glycerol kinase 5	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	the mRNA and protein levels of GK5 are significantly upregulated in gefitinib-resistant human lung adenocarcinoma PC9R and H1975 cells compared with gefitinib-sensitive PC9 cells	up

General Information

General Information	Comment	Organism
malfunction	silencing GK5 in PC9R cells induces mitochondrial damage, caspase activation, cell cycle arrest, and apoptosis via SREBP1/SCD1 signaling pathway. The exosomal mRNA of GK5 in the plasma of patients with gefitinib-resistant adenocarcinoma is significantly higher compared with that of gefitinib-sensitive patients. GK5 knockdown induces PC9R cell apoptosis and cell cycle arrest in the presence of gefitinib, as well as PC9R cell mitochondrial dysfunction and caspase activation. GK5 knockdown suppresses tumor proliferation in vivo	Homo sapiens
physiological function	GK5 confers gefitinib resistance in lung cancer by inhibiting apoptosis and cell cycle arrest. Role of glycerol kinase 5 (GK5) in mediating gefitinib resistance in non-small cell lung cancer (NSCLC), overview. GK5 confers gefitinib resistance through upregulating SCD1 expression, molecular mechanism underlying GK5-mediated gefitinib resistance	Homo sapiens

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Release 2023.1 (January 2023)