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Literature summary for 2.7.1.30 extracted from

  • Zhang, C.; Meng, X.; Gu, H.; Ma, Z.; Lu, L.
    Predicted glycerol 3-phosphate dehydrogenase homologs and the glycerol kinase GlcA coordinately adapt to various carbon sources and osmotic stress in Aspergillus fumigatus (2018), G3 (Bethesda), 8, 2291-2299 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene glcA, quantitative RT-PCR expression analysis Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
additional information knockout glcA in the DELTAgfdA and the parental wild-type strain backgrounds. The glcA null mutant shows defects of growth and conidiation in glycerol media but not in glucose media, suggesting glcA is required when glycerol is the sole carbon source, and gfdA is required when glucose is the sole carbon source. The DELTAgfdADELTAglcA double mutant shows an exacerbation of colony defects in both glucose and glycerol media. In glucose medium, DELTAgfdADELTAglcA colonies are very small, and colonies are nearly undetectable colonies in the glycerol medium. Transformation of the full-length ORF sequence of glcA under the control of the constitutive promoter gpdA into the DELTAgfdA and reference strains, resulting in two glcA-overexpression strains, DELTAgfdAOE::glcA and wild-type OE::glcA. Overexpressed glcA is able to rescue growth defects associated with loss of gfdA. In comparison, wild-type OE::glcA still shows wild-type like colony phenotypes. Overexpression of glcA may result in the production of accumulated glycerol 3-phosphate (G3P), which allows colonies to bypass the requirement of gfdA to produce glycerol use in colony growth, indicating that the growth defects of gfdA null mutant might be due to absence of G3P rather than glycerol. In contrast, when glycerol is used as the sole carbon source, DELTAgfdAOE::glcA shows defective phenotypes similar to wild-type OE::glcA, indicating the overexpression of glcA may cause the production of accumulated G3P, which results in growth defects in glycerol media either in the presence or absence of gfdA Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycerol Aspergillus fumigatus
-
ADP + sn-glycerol 3-phosphate
-
?
ATP + glycerol Aspergillus fumigatus CBS 144.89
-
ADP + sn-glycerol 3-phosphate
-
?
ATP + glycerol Aspergillus fumigatus CEA10
-
ADP + sn-glycerol 3-phosphate
-
?
ATP + glycerol Aspergillus fumigatus FGSC A1163
-
ADP + sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus B0Y6Y4 Aspergillus fumigatus
-
Aspergillus fumigatus CBS 144.89 B0Y6Y4 Aspergillus fumigatus
-
Aspergillus fumigatus CEA10 B0Y6Y4 Aspergillus fumigatus
-
Aspergillus fumigatus FGSC A1163 B0Y6Y4 Aspergillus fumigatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol
-
Aspergillus fumigatus ADP + sn-glycerol 3-phosphate
-
?
ATP + glycerol
-
Aspergillus fumigatus CBS 144.89 ADP + sn-glycerol 3-phosphate
-
?
ATP + glycerol
-
Aspergillus fumigatus CEA10 ADP + sn-glycerol 3-phosphate
-
?
ATP + glycerol
-
Aspergillus fumigatus FGSC A1163 ADP + sn-glycerol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
AFUB_068560
-
Aspergillus fumigatus
glcA
-
Aspergillus fumigatus

General Information

General Information Comment Organism
malfunction overexpression of glycerol kinase GlcA bypasses the requirement of glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), encoded by gene gfdA, in glucose media for colony growth. The DELTAgfdADELTAglcA double mutant shows an exacerbation of colony defects in both glucose and glycerol media Aspergillus fumigatus
metabolism glycerol 3-phosphate (G3P) can be synthesized by two classical pathways. The first pathway is catalyzed by an NAD-dependent glycerol 3-phosphate dehydrogenase converting dihydroxyacetone phosphate (DHAP) into G3P The second pathway is catalyzed by a glycerol kinase encoded by glcA converting glycerol to G3P Aspergillus fumigatus
physiological function glycerol plays an important role in the adaptation of fungi to various microenvironments and stressors, including heat shock, anoxic conditions and osmotic stress. Glycerol kinase GlcA coordinately adapts to various carbon sources and osmotic stress in Aspergillus fumigatus. It is suggested that glcA is required when glycerol is the sole carbon source, and gfdA is required when glucose is the sole carbon source, functional relationship between gfdA and glcA, overview Aspergillus fumigatus