Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 5 mM, 60% inhibition | [Candida] methylica | |
Mn2+ | MnCl2 | [Candida] methylica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
dihydroxyacetone | pH 7.5, 30°C | [Candida] methylica | |
0.024 | - |
DL-glyceraldehyde | pH 7.5, 30°C | [Candida] methylica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mg2+ in activation, 30.3% of activity with Mg2+ | [Candida] methylica | |
Co2+ | can partially replace Mg2+ in activation, 57.3% of activity with Mg2+ | [Candida] methylica | |
Mg2+ | divalent cation required, highest activity with Mg2+ | [Candida] methylica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | [Candida] methylica | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | ADP + dihydroxyacetone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
- |
- |
Ogataea angusta CBS 4732 | - |
- |
- |
[Candida] methylica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ogataea angusta |
streptomycin sulfate, ammonium sulfate, Cibacron blue F3G-ASephadex g-200, DEAE cellulose, partially purified | [Candida] methylica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.65 | - |
- |
[Candida] methylica |
Storage Stability | Organism |
---|---|
-20°C, 60% glycerol, at least 4 weeks, no loss of activity | [Candida] methylica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydroxyacetone | - |
Ogataea angusta | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | - |
[Candida] methylica | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | second step in assimilation of formaldehyde via the xylulose monophosphate, i.e. dihydroxyacetone cycle during growth of yeast on methanol | [Candida] methylica | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + dihydroxyacetone | - |
Ogataea angusta CBS 4732 | ADP + dihydroxyacetone phosphate | - |
? | |
ATP + DL-glyceraldehyde | 25% of the activity with dihydroxyacetone | [Candida] methylica | ADP + glyceraldehyde 3-phosphate | - |
? | |
CTP + dihydroxyacetone | lower than 1% of the activity with ATP | [Candida] methylica | CDP + dihydroxyacetone phosphate | - |
? | |
GTP + dihydroxyacetone | lower than 1% of the activity with ATP | [Candida] methylica | GDP + dihydroxyacetone phosphate | - |
? | |
ITP + dihydroxyacetone | 11.2% of activity with ATP | [Candida] methylica | IDP + dihydroxyacetone phosphate | - |
? | |
UTP + dihydroxyacetone | 3.1% of the activity with ATP | [Candida] methylica | UDP + dihydroxyacetone phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | [Candida] methylica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
[Candida] methylica |