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Literature summary for 2.7.1.25 extracted from

  • Wang, D.; Xu, S.; Song, D.; Knight, S.; Mao, X.
    A gene encoding a potential adenosine 5'-phosphosulphate kinase is necessary for timely development of Myxococcus xanthus (2016), Microbiology, 162, 672-683 .
    View publication on PubMed
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
gene MXAN3487 or cysC, DNA and amino acid sequence determination and analysis, sequence comparisons, the gene is encoded in the mcuABC, an operon coding for part of the chaperone-usher (CU) system Myxococcus xanthus

Protein Variants

Protein Variants Comment Organism
K19R site-directed mutagenesis, the P-loop Lys is substituted by Arg. K19R is a kinase-dead point mutation, because the Lys residue in the P-loop is required for productive ATP binding and kinase activity Myxococcus xanthus
additional information construction of MXAN3487-lacZ fusion protein. Genome-wide transposon mutagenesis is used to identify MXAN3487 (MXAN_RS16905) as a gene encoding a putative adenosine 5'-phosphosulfate (APS) kinase (i.e. adenylylsulfate kinase), as a regulator for the timing of cell aggregation. In-frame deletion resulting in plasmid pBJ113-DELTAMXAN3487, a pBJ113 derivative. Expression of the Escherichia coli cysC gene in mutant strain DELTAMXAN3487 partially complements the phenotype Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus Q1D6P1
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Myxococcus xanthus DK 1622 Q1D6P1
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Synonyms

Synonyms Comment Organism
adenosine 5'-phosphosulphate kinase
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 Myxococcus xanthus
adenylylsulphate kinase
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 Myxococcus xanthus
APS kinase
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 Myxococcus xanthus
CysC
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 Myxococcus xanthus
MXAN3487
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 Myxococcus xanthus
MXAN_3487
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 Myxococcus xanthus
MXAN_RS16905 locus name Myxococcus xanthus

Expression

Organism Comment Expression
Myxococcus xanthus expression of gene MXAN3487 is not affected by sulfate availability additional information
Myxococcus xanthus expression of MXAN3487 is induced during development and depends on FruA a key response regulator protein in the C-signalling pathway (protein-NPi-phosphohistidine-D-fructose phosphotransferase, EC 2.7.1.202). Aggregation defect of DMXAN3487, the K19R allele is unable to rescue the sporulation defect of DELTAMXAN3487. Mutant phenotype overview up

General Information

General Information Comment Organism
evolution the MXAN3487 protein displays sequence and structural homology to adenosine 5'-phosphosulfate (APS) kinase family members and contains putative motifs for ATP and APS binding Myxococcus xanthus
malfunction deletion of gene MXAN3487 significantly delays fruiting body formation and the production of McuA, a spore coat protein secreted by the Myxococcus xanthus Mcu CU system. Delayed expression of mcuABC in DELTAMXAN3487 may be secondary to a cell aggregation defect Myxococcus xanthus
metabolism APS kinase catalyses the second reaction in the two-step conversion of inorganic sulphate to 3'-phosphoadenosine 5'-phosphosulfate (PAPS). MXAN3487 is not regulated by sulfur starvation Myxococcus xanthus
additional information MXAN3487 protein homology modeling Myxococcus xanthus
physiological function although the MXAN3487 locus is not linked to other sulfate assimilation genes, its protein product may have adenosine 5'-phosphosulfate (APS) kinase activity in vivo. The importance of the ATP-binding site for activity is demonstrated. MXAN3487 seems not to function in a reductive sulfate assimilation pathway. The enzyme may phosphorylate molecules structurally related to APS, generating metabolites necessary for Myxococcus xanthus development, and exerts a positive effect on the mcuABC operon whose expression is morphogenesis-dependent. The enzyme is a regulator for the timing of cell aggregation. Protein MXAN3487 is a bona fide APS kinase, the kinase activity of MXAN3487 protein is essential for its function. MXAN3487 is not regulated by sulfur starvation Myxococcus xanthus