Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | high salt activates at high adenosine 5'-phosphosulfate concentrations but inhibits at low adenosine 5'-phosphosulfate concentrations | Penicillium chrysogenum |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and S107A mutant enzyme in Escherichia coli | Penicillium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
S104A | similar properties as wild-type | Penicillium chrysogenum |
S107A | similar properties as wild-type enzyme | Penicillium chrysogenum |
S107C | similar properties as wild-type, suggesting that S107 is not essential for activity but may be located in the substrate binding pocket | Penicillium chrysogenum |
S97A | similar properties as wild-type | Penicillium chrysogenum |
S99A | similar properties as wild-type | Penicillium chrysogenum |
T103A | similar properties as wild-type | Penicillium chrysogenum |
Y109F | similar properties as wild-type, velocity curve is shifted to the far right | Penicillium chrysogenum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | high salt inhibits at low adenosine 5'-phosphosulfate concentrations, but activates at high adenosine 5'-phosphosulfate concentrations | Penicillium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
adenosine 5'-phosphosulfate | pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate | Penicillium chrysogenum | |
0.003 | - |
3'-phosphoadenosine 5'-phosphosulfate | pH 8.1, 30°C, wild-type enzyme | Penicillium chrysogenum | |
0.012 | - |
adenosine 5'-phosphosulfate | pH 8.1, 30°C, S107A mutant enzyme at 100 mM ammonium sulfate | Penicillium chrysogenum | |
0.039 | - |
3'-phosphoadenosine 5'-phosphosulfate | pH 8.1, 30°C, S107A mutant enzyme | Penicillium chrysogenum | |
0.8 | - |
ATP | pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate | Penicillium chrysogenum | |
0.8 | - |
ATP | ATP in form of MgATP2- | Penicillium chrysogenum | |
2.4 | - |
ATP | pH 8.1, 30°C, S107A mutant enzyme | Penicillium chrysogenum | |
2.4 | - |
ATP | ATP in form of MgATP2- | Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P23846 | - |
- |
Penicillium chrysogenum | Q12657 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Penicillium chrysogenum | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Penicillium chrysogenum | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Escherichia coli | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
ATP + adenylyl sulfate | - |
Penicillium chrysogenum | ADP + 3'-phosphoadenylyl sulfate | - |
r |