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Literature summary for 2.7.1.25 extracted from

  • MacRae, I.J.; Rose, A.B.; Segel, I.H.
    Adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum. site-directed mutagenesis at putative phosphoryl-accepting and ATP P-loop residues (1998), J. Biol. Chem., 273, 28583-28589.
    View publication on PubMed
Search for more literature for 2.7.1.25

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate high salt activates at high adenosine 5'-phosphosulfate concentrations but inhibits at low adenosine 5'-phosphosulfate concentrations Penicillium chrysogenum

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and S107A mutant enzyme in Escherichia coli Penicillium chrysogenum

Protein Variants

Protein Variants Comment Organism
S104A similar properties as wild-type Penicillium chrysogenum
S107A similar properties as wild-type enzyme Penicillium chrysogenum
S107C similar properties as wild-type, suggesting that S107 is not essential for activity but may be located in the substrate binding pocket Penicillium chrysogenum
S97A similar properties as wild-type Penicillium chrysogenum
S99A similar properties as wild-type Penicillium chrysogenum
T103A similar properties as wild-type Penicillium chrysogenum
Y109F similar properties as wild-type, velocity curve is shifted to the far right Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
ammonium sulfate high salt inhibits at low adenosine 5'-phosphosulfate concentrations, but activates at high adenosine 5'-phosphosulfate concentrations Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 adenosine 5'-phosphosulfate pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate Penicillium chrysogenum
0.003
-
 3'-phosphoadenosine 5'-phosphosulfate pH 8.1, 30°C, wild-type enzyme Penicillium chrysogenum
0.012
-
 adenosine 5'-phosphosulfate pH 8.1, 30°C, S107A mutant enzyme at 100 mM ammonium sulfate Penicillium chrysogenum
0.039
-
 3'-phosphoadenosine 5'-phosphosulfate pH 8.1, 30°C, S107A mutant enzyme Penicillium chrysogenum
0.8
-
 ATP pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate Penicillium chrysogenum
0.8
-
 ATP ATP in form of MgATP2- Penicillium chrysogenum
2.4
-
 ATP pH 8.1, 30°C, S107A mutant enzyme Penicillium chrysogenum
2.4
-
 ATP ATP in form of MgATP2- Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Escherichia coli P23846
-
-
Penicillium chrysogenum Q12657
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenylyl sulfate
-
 Penicillium chrysogenum ADP + 3'-phosphoadenylyl sulfate
-
 r