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Literature summary for 2.7.1.25 extracted from

  • Onda, M.; Hayashi, M.; Suiko, M.; Liu, M.C.; Nakajima, H.
    Purification and characterization of adenosine 5'-phosphosulfate kinase from the thermophilic bacterium Bacillus stearothermophilus (1996), Biosci. Biotechnol. Biochem., 60, 134-136.
No PubMed abstract available
Search for more literature for 2.7.1.25

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by adenosine 5'-phosphosulfate Geobacillus stearothermophilus
additional information
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Geobacillus stearothermophilus
0.22
-
 ATP pH 8.0, 30°C Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 164% of activation with Mg2+ Geobacillus stearothermophilus
Mg2+
-
 Geobacillus stearothermophilus
Mn2+ 274% of activation with Mg2+ Geobacillus stearothermophilus
Zn2+ 309% of activation with Mg2+ Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Geobacillus stearothermophilus
50000
-
 gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, Matrex gel blue A, phenyl Cellofine Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Saccharomyces cerevisiae ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Geobacillus stearothermophilus ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae
dimer 2 * 26000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 no loss of activity after 7 d at room temperatures Geobacillus stearothermophilus
25
-
 70% loss of activity after 7 d at room temperature Saccharomyces cerevisiae
60
-
 complete inactivation after 15 min Saccharomyces cerevisiae
70
-
 no significant loss of activity after 15 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7
-
 Saccharomyces cerevisiae
7 8
-
 Geobacillus stearothermophilus