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Literature summary for 2.7.1.25 extracted from

  • Renosto, F.; Schultz, T.; Re, E.; Mazer, J.; Chandler, C.J.; Barron, A.; Segel, I.H.
    Comparative stability and catalytic and chemical properties of the sulfate-activating enzymes from Penicillium chrysogenum (mesophile) and Penicillium duponti (thermophile) (1985), J. Bacteriol., 164, 674-683.
    View publication on PubMedView publication on EuropePMC
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Penicillium chrysogenum
Mg2+ activation Penicillium duponti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C Penicillium chrysogenum
57000
-
 gel filtration at 22°C Penicillium chrysogenum
57000
-
 gel filtration at 22°C or 46°C Penicillium duponti

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-
Penicillium duponti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium chrysogenum
partial Penicillium duponti

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Penicillium chrysogenum
-
mycelium
-
 Penicillium duponti
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium duponti ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r

Subunits

Subunits Comment Organism
dimer 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 inactivation above, reversible by cooling to 0-30°C Penicillium chrysogenum