Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.25 extracted from

  • Renosto, F.; Seubert, P.A.; Segel, I.H.
    Adenosine 5-phosphosulfate kinase from Penicillium chrysogenum. Purification and kinetic characterization (1984), J. Biol. Chem., 259, 2113-2123.
    View publication on PubMed
Search for more literature for 2.7.1.25

Inhibitors

Inhibitors Comment Organism Structure
2'-Phosphoadenosine 5'-phosphosulfate
-
 Penicillium chrysogenum
3'-phosphoadenosine 5'-phosphosulfate product inhibition Penicillium chrysogenum
adenosine 5'-phosphosulfate kinetics Penicillium chrysogenum
ATP inhibition by free ATP, i.e. in excess of total Mg2+ Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Penicillium chrysogenum
0.0014
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Penicillium chrysogenum
0.008
-
 3'-phosphoadenosine 5'-phosphosulfate pH 8.0, 30°C Penicillium chrysogenum
1.5
-
 ATP pH 8.0, 30°C Penicillium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 58% of activation with Mg2+ or Mn2+ Penicillium chrysogenum
Mg2+ activation Penicillium chrysogenum
Mn2+ 5 mM yield the same reaction velocity as 5 mM Mg2+, excess inhibits slightly Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Penicillium chrysogenum
59000 60000 gel filtration Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine 5-phosphosulfate Penicillium chrysogenum second step in pathway of assimilation of inorganic sulfate ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Affi-gel blue, Matrix-gel green, Agarose A Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.7
-
-
 Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
ATP + adenosine 5-phosphosulfate second step in pathway of assimilation of inorganic sulfate Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 rapid loss of activity, approx. 80% of activity is recovered upon cooling at 0°C, presence of MgATP2- accelerates the recovery process Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 90% of maximal activity at pH 7.5 and pH 8.5 Penicillium chrysogenum