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Literature summary for 2.7.1.24 extracted from
- Identification of critical residues of the mycobacterial dephosphocoenzyme A kinase by site-directed mutagenesis (2011), PLoS ONE, 6, e15228.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D32A | the mutant shows a catalytic efficiency of only 5% of the native enzyme | Mycobacterium tuberculosis |
| D32E | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
| D32N | the mutant shows an almost 2.54times increased Km value for 3'-dephospho-CoA compared to the wild type enzyme. The absence of magnesium completely ablates activity for the D32N mutant | Mycobacterium tuberculosis |
| G8A | this mutation does not change either the Km or the Kcat of the reaction considerably | Mycobacterium tuberculosis |
| K14A | the substitution affects the kinetic parameters of the reaction resulting in a mere 19% reduction in the Kcat of the enzyme, the mutant demonstrates a 50% increase in the Km for ATP | Mycobacterium tuberculosis |
| L114A | the mutation results in a decrease in the affinity of the enzyme for the acceptor substrate | Mycobacterium tuberculosis |
| R140A | this mutation completely abolishes kinetic activity | Mycobacterium tuberculosis |
| R140K | the mutation results in a dramatic loss of catalytic activity | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0217 | - |
3'-dephospho-CoA | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis |  |
| 0.0345 | - |
ATP | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0349 | - |
3'-dephospho-CoA | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0372 | - |
3'-dephospho-CoA | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0501 | - |
ATP | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0568 | - |
ATP | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0575 | - |
ATP | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0598 | - |
ATP | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0639 | - |
ATP | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0758 | - |
3'-dephospho-CoA | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0809 | - |
3'-dephospho-CoA | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0867 | - |
ATP | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0877 | - |
3'-dephospho-CoA | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.181 | - |
3'-dephospho-CoA | mutant enzyme R140K, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.501 | - |
3'-dephospho-CoA | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WPA3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3'-dephospho-CoA | - |
Mycobacterium tuberculosis | ADP + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CoaE | - |
Mycobacterium tuberculosis |
| dephosphocoenzyme A kinase | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
3'-dephospho-CoA | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0076 | - |
3'-dephospho-CoA | mutant enzyme R140K, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.014 | - |
ATP | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0192 | - |
ATP | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0255 | - |
3'-dephospho-CoA | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0257 | - |
3'-dephospho-CoA | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0293 | - |
3'-dephospho-CoA | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0327 | - |
3'-dephospho-CoA | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0334 | - |
3'-dephospho-CoA | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.039 | - |
ATP | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.043 | - |
ATP | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0481 | - |
ATP | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0569 | - |
3'-dephospho-CoA | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0651 | - |
ATP | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.067 | - |
ATP | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00034 | - |
3'-dephospho-CoA | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.0015 | - |
3'-dephospho-CoA | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.042 | - |
3'-dephospho-CoA | mutant enzyme R140K, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.243 | - |
ATP | mutant enzyme D32A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.3 | - |
ATP | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.337 | - |
3'-dephospho-CoA | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.38 | - |
3'-dephospho-CoA | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.448 | - |
ATP | mutant enzyme K14A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.69 | - |
3'-dephospho-CoA | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.703 | - |
3'-dephospho-CoA | mutant enzyme L114A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.713 | - |
ATP | mutant enzyme G8A, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.838 | - |
3'-dephospho-CoA | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 0.847 | - |
ATP | wild type enzyme, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 1.3 | - |
ATP | mutant enzyme D32N, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
| 1.93 | - |
ATP | mutant enzyme D32E, in 50 mM Tris with MgCl2 (10 mM) and KCl (20 mM), pH 8.0, at 25°C | Mycobacterium tuberculosis | |
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