Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-RIL cells | Xanthomonas oryzae pv. oryzicola |
Protein Variants | Comment | Organism |
---|---|---|
D193N | catalytically impaired mutant | Xanthomonas oryzae pv. oryzicola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AvrRxo2 | potent, mixed inhibitor of the enzyme | Xanthomonas oryzae pv. oryzicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | with NAAD+ or NAD+ as cosubstrate, at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
1.2 | - |
NAD+ | at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
1.2 | - |
NaAD+ | at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 3 mM used in assay conditions | Xanthomonas oryzae pv. oryzicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAAD+ | Xanthomonas oryzae pv. oryzicola | NAAD+ is nicotinic acid adenine dinucleotide | ADP + 3'-NAADP+ | - |
? | |
ATP + NAD+ | Xanthomonas oryzae pv. oryzicola | - |
ADP + 3'-NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae pv. oryzicola | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, HiTrap heparin column chromatography, and Superdex 75 gel filtration | Xanthomonas oryzae pv. oryzicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAAD+ | NAAD+ is nicotinic acid adenine dinucleotide | Xanthomonas oryzae pv. oryzicola | ADP + 3'-NAADP+ | - |
? | |
ATP + NAD+ | - |
Xanthomonas oryzae pv. oryzicola | ADP + 3'-NADP+ | - |
? | |
GTP + NAD+ | - |
Xanthomonas oryzae pv. oryzicola | GDP + 3'-NADP+ | - |
? | |
additional information | the enzyme accepts neither GDP nor GTP as phosphate acceptors | Xanthomonas oryzae pv. oryzicola | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
AvrRxo1 | - |
Xanthomonas oryzae pv. oryzicola |
NAD/NAAD kinase | - |
Xanthomonas oryzae pv. oryzicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
270 | - |
NaAD+ | at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
340 | - |
ATP | with NAAD+ as cosubstrate, at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
430 | - |
NAD+ | at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
460 | - |
ATP | with NAD+ as cosubstrate, at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
225 | - |
NaAD+ | at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
340 | - |
ATP | with NAAD+ as cosubstrate, at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
358 | - |
NAD+ | at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola | |
460 | - |
ATP | with NAD+ as cosubstrate, at pH 7.5 and 25°C | Xanthomonas oryzae pv. oryzicola |