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Literature summary for 2.7.1.236 extracted from

  • Schuebel, F.; Rocker, A.; Edelmann, D.; Schessner, J.; Brieke, C.; Meinhart, A.
    3-NADP and 3-NAADP, two metabolites formed by the bacterial type III effector AvrRxo1 (2016), J. Biol. Chem., 291, 22868-22880 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.236

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-RIL cells Xanthomonas oryzae pv. oryzicola

Protein Variants

Protein Variants Comment Organism
D193N catalytically impaired mutant Xanthomonas oryzae pv. oryzicola

Inhibitors

Inhibitors Comment Organism Structure
AvrRxo2 potent, mixed inhibitor of the enzyme Xanthomonas oryzae pv. oryzicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 ATP with NAAD+ or NAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
1.2
-
 NAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
1.2
-
 NaAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 3 mM used in assay conditions Xanthomonas oryzae pv. oryzicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NAAD+ Xanthomonas oryzae pv. oryzicola NAAD+ is nicotinic acid adenine dinucleotide ADP + 3'-NAADP+
-
 ?
ATP + NAD+ Xanthomonas oryzae pv. oryzicola
-
 ADP + 3'-NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas oryzae pv. oryzicola
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HiTrap heparin column chromatography, and Superdex 75 gel filtration Xanthomonas oryzae pv. oryzicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAAD+ NAAD+ is nicotinic acid adenine dinucleotide Xanthomonas oryzae pv. oryzicola ADP + 3'-NAADP+
-
 ?
ATP + NAD+
-
 Xanthomonas oryzae pv. oryzicola ADP + 3'-NADP+
-
 ?
GTP + NAD+
-
 Xanthomonas oryzae pv. oryzicola GDP + 3'-NADP+
-
 ?
additional information the enzyme accepts neither GDP nor GTP as phosphate acceptors Xanthomonas oryzae pv. oryzicola ?
-
 ?

Synonyms

Synonyms Comment Organism
AvrRxo1
-
 Xanthomonas oryzae pv. oryzicola
NAD/NAAD kinase
-
 Xanthomonas oryzae pv. oryzicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
270
-
 NaAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
340
-
 ATP with NAAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
430
-
 NAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
460
-
 ATP with NAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
225
-
 NaAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
340
-
 ATP with NAAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
358
-
 NAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
460
-
 ATP with NAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola