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Literature summary for 2.7.1.235 extracted from

  • Kreamer, N.; Chopra, R.; Caughlan, R.; Fabbro, D.; Fang, E.; Gee, P.; Hunt, I.; Li, M.; Leon, B.; Muller, L.; Vash, B.; Woods, A.; Stams, T.; Dean, C.; Uehara, T.
    Acylated-acyl carrier protein stabilizes the Pseudomonas aeruginosa WaaP lipopolysaccharide heptose kinase (2018), Sci. Rep., 8, 14124 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.235

Activating Compound

Activating Compound Comment Organism Structure
myristoyl-ACP myristoyl-ACP is the likely physiological binding partner for WaaP Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. WaaP produced by in vitro transcription-translation is insoluble unless acyl-ACP is present Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
structure in complex with acylated-acyl carrier protein (acyl-ACP) Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
A214F mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
A214L mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
A214W mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
D163A mutation in HRD motif, no growth in an EDTA resistance assay Pseudomonas aeruginosa
D188N mutation in ATP binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
E78A mutation in ATP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
H161A mutation in HRD motif, no growth in an EDTA resistance assay Pseudomonas aeruginosa
H168A mutation in substrate binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
K51A mutation in ATP binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
K69A mutation in substrate binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
L143W mutation in lipid terminus, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
L219W mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
L228A mutation in pocket proximal to phosphopentatheine, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
L228W mutation in pocket proximal to phosphopentatheine, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
additional information WaaP produced by in vitro transcription-translation is insoluble unless acyl-ACP is present. WaaP variants designed to perturb the acyl-ACP interaction are less stable in cells and exhibit reduced kinase function Pseudomonas aeruginosa
R162A mutation in HRD motif, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R191A mutation in substrate binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
R221E/R222E/R226E/R229E/R237E mutation in ACP binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
R222A mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R222A/R226A mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R222E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R222E/R226E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R222E/R226E/R237E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R226A mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
R226E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
S127L mutation in lipid terminus, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
T198A mutation in phosphorylation site, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
V147L mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
V147L/L219W mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
V147W mutation in mid pocket, no growth in an EDTA resistance assay Pseudomonas aeruginosa
V147W/214F mutation in mid pocket, no growth in an EDTA resistance assay Pseudomonas aeruginosa
Y165A mutation in substrate binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HUF7 cf. EC 2.7.10.2
-
Pseudomonas aeruginosa DSM 22644 Q9HUF7 cf. EC 2.7.10.2
-

Synonyms

Synonyms Comment Organism
RfaP
-
 Pseudomonas aeruginosa
WaaP
-
 Pseudomonas aeruginosa