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Literature summary for 2.7.1.232 extracted from

  • Xie, H.; Zhuang, X.; Zhang, H.; Bai, Z.; Qi, H.
    Screening and identification of the levoglucosan kinase gene (lgk) from Aspergillus niger by LC-ESI-MS/MS and RT-PCR (2005), FEMS Microbiol. Lett., 251, 313-319.
    View publication on PubMed
Search for more literature for 2.7.1.232

Cloned(Commentary)

Cloned (Comment) Organism
gene lgk, DNA and amino acid seuence determination and analysis, sequence comparisons, recombinant expression in Saccharomyces cerevisiae strain H158 from pasmid pAJ401 Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + levoglucosan + H2O Aspergillus niger
-
 ADP + D-glucopyranose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 gene lgk
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by ammonium sulfate fractionation and anion exchange chromatography Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + levoglucosan + H2O
-
 Aspergillus niger ADP + D-glucopyranose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
LGK
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Aspergillus niger