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Literature summary for 2.7.1.23 extracted from

  • Gelin, M.; Poncet-Montange, G.; Assairi, L.; Morellato, L.; Huteau, V.; Dugue, L.; Dussurget, O.; Pochet, S.; Labesse, G.
    Screening and in situ synthesis using crystals of a NAD kinase lead to a potent antistaphylococcal compound (2012), Structure, 20, 1107-1117.
    View publication on PubMed
Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Listeria monocytogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme bound to compounds 5'-O-propargyl-5'-deoxyadenosine, 5'-azido-5'-deoxyadenosine, 8-bromo-5'-amino-5'-deoxyadenosine, or 8-thioglycolic-5'-azido-5'-deoxyadenosine, using 30 mM sodium bromide, 220 mM potassium citrate, pH 4.8-5.1, 6% glycerol, 15%-16% (w/v) polyethylene glycol 400 Listeria monocytogenes

Inhibitors

Inhibitors Comment Organism Structure
2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide
-
 Listeria monocytogenes

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes Q8Y8D7
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt-based IMAC resin column chromatography Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Listeria monocytogenes ADP + NADP+
-
 ?
additional information the NAD kinase from Listeria monocytogenes can promote amide formation between 5'-amino-5'-deoxyadenosine and carboxylic acid groups Listeria monocytogenes ?
-
 ?

Synonyms

Synonyms Comment Organism
NAD kinase
-
 Listeria monocytogenes
NADK1
-
 Listeria monocytogenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
 2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide in 50 mM Tris-HCl, pH 7.4, 10 mM MgCl2, at 30°C Listeria monocytogenes