Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
0.4 | - |
ATP | with NADH as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
1.59 | - |
NAD+ | in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
1.74 | - |
ATP | with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 43% activity at 5 mM compared to Mg2+ | Entamoeba histolytica | |
Co2+ | 36% activity at 5 mM compared to Mg2+ | Entamoeba histolytica | |
Cu2+ | 2% activity at 5 mM compared to Mg2+ | Entamoeba histolytica | |
Fe2+ | 83% activity at 5 mM compared to Mg2+ | Entamoeba histolytica | |
Mg2+ | preferred cation, 100% activity at 5 mM | Entamoeba histolytica | |
Mn2+ | 15% activity at 5 mM compared to Mg2+ | Entamoeba histolytica | |
additional information | no activity is detected in presence of Li+, Na+, and K+ | Entamoeba histolytica | |
Ni2+ | 6% activity at 5 mM compared to Mg2+ | Entamoeba histolytica | |
Zn2+ | 10% activity at 5 mM compared to Mg2+ | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
x * 29500, calculated from amino acid sequence | Entamoeba histolytica |
32000 | - |
x * 32000, recombinant enzyme, SDS-PAGE | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | C4LSN1 | - |
- |
Entamoeba histolytica HM1: IMSS cl 6 | C4LSN1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, gel filtration | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
-30 or -80°C, 10-15% (v/v) glycerol, at least 3 months, no loss of activity | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + NADH | 13% activity compared to ATP | Entamoeba histolytica | AMP + NADPH | - |
? | |
ATP + NAD+ | 50% activity compared to NADH | Entamoeba histolytica | ADP + NADP+ | - |
? | |
ATP + NAD+ | 50% activity compared to NADH | Entamoeba histolytica HM1: IMSS cl 6 | ADP + NADP+ | - |
? | |
ATP + NADH | 100% activity | Entamoeba histolytica | ADP + NADPH | - |
? | |
ATP + NADH | 100% activity | Entamoeba histolytica HM1: IMSS cl 6 | ADP + NADPH | - |
? | |
CTP + NADH | 74% activity compared to ATP | Entamoeba histolytica | CDP + NADPH | - |
? | |
CTP + NADH | 74% activity compared to ATP | Entamoeba histolytica HM1: IMSS cl 6 | CDP + NADPH | - |
? | |
dATP + NADH | 103% activity compared to ATP | Entamoeba histolytica | dADP + NADPH | - |
? | |
GTP + NADH | 102% activity compared to ATP | Entamoeba histolytica | GDP + NADPH | - |
? | |
additional information | the enzyme does not use AMP, CMP or inorganic polyphosphates (diphosphate, tripolyphosphate, trimetaphosphate, hexametaphosphate, metaphosphate, and polyphosphate) as the phosphoryl donor. Glucose 6-phosphate and phosphoenolpyruvate are also inert as phosphoryl donors | Entamoeba histolytica | ? | - |
? | |
additional information | the enzyme does not use AMP, CMP or inorganic polyphosphates (diphosphate, tripolyphosphate, trimetaphosphate, hexametaphosphate, metaphosphate, and polyphosphate) as the phosphoryl donor. Glucose 6-phosphate and phosphoenolpyruvate are also inert as phosphoryl donors | Entamoeba histolytica HM1: IMSS cl 6 | ? | - |
? | |
TTP + NADH | 50% activity compared to ATP | Entamoeba histolytica | TDP + NADPH | - |
? | |
UTP + NADH | 69% activity compared to ATP | Entamoeba histolytica | UDP + NADPH | - |
? | |
UTP + NADH | 69% activity compared to ATP | Entamoeba histolytica HM1: IMSS cl 6 | UDP + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant enzyme, SDS-PAGE | Entamoeba histolytica |
? | x * 29500, calculated from amino acid sequence | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
NAD(H) kinase | - |
Entamoeba histolytica |
NADHK | - |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
NAD+ | in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
1.9 | - |
NADH | in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
2.02 | - |
ATP | with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
2.68 | - |
ATP | with NADH as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | calculated from amino acid sequence | - |
6.5 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme overexpression represses the production of intracellular reactive oxygen specie upon H2O2 exposure by 40% | Entamoeba histolytica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.16 | - |
ATP | with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
1.2 | - |
NAD+ | in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
6.72 | - |
ATP | with NADH as cosubstrate, in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica | |
56.48 | - |
NADH | in 100 mM Tris-HCl (pH 7.5), at 37°C | Entamoeba histolytica |