Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.23 extracted from

  • Kawai, S.; Fukuda, C.; Mukai, T.; Murata, K.
    MJ0917 in archaeon Methanococcus jannaschii is a novel NADP phosphatase/NAD kinase (2005), J. Biol. Chem., 280, 39200-39207.
    View publication on PubMed
Search for more literature for 2.7.1.23

Activating Compound

Activating Compound Comment Organism Structure
5'-AMP 1.56fold activation at 3.0 mM Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
ADP 3 mM, 29% inhibition Methanocaldococcus jannaschii
NADP+ 0.3 mM, complete inhibition Methanocaldococcus jannaschii
NADPH 0.3 mM, 18% inhibition Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 ATP 85°C, pH 8.5, 20 mM Mg2+ Methanocaldococcus jannaschii
3
-
 NAD+ 85°C, pH 8.5, 20 mM Mg2+ Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity is dependent on Mg2+, maximal activity at 50 mM Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
99.4
-
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Methanocaldococcus jannaschii ADP + NADP+
-
 ?
ATP + NADH 14% of the activity NAD+ Methanocaldococcus jannaschii ADP + NADPH
-
 ?
CTP + NAD+ 102% of the activity with ATP Methanocaldococcus jannaschii CDP + NADP+
-
 ?
dATP + NAD+ 73% of the activity with ATP Methanocaldococcus jannaschii dADP + NADP+
-
 ?
GTP + NAD+ 66% of the activity with ATP Methanocaldococcus jannaschii GDP + NADP+
-
 ?
hexametaphosphate + NAD+ 47% of the activity with ATP Methanocaldococcus jannaschii NADP+ + ?
-
 ?
additional information the enzyme also shows phosphatase activity Methanocaldococcus jannaschii ?
-
 ?
TTP + NAD+ 71% of the activity with ATP Methanocaldococcus jannaschii TDP + NADP+
-
 ?
UTP + NAD+ 114% of the activity with ATP Methanocaldococcus jannaschii UDP + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
MJ0917
-
 Methanocaldococcus jannaschii
NADP phosphatase/NAD kinase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 100 80°C: about 45% of maximal activity, 100°C: optimum Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
399
-
 ATP 85°C, pH 8.5, 20 mM Mg2+ Methanocaldococcus jannaschii
424
-
 NAD+ 85°C, pH 8.5, 20 mM Mg2+ Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 35% of maximal activity, pH 9.5: about 90% of maximal activity Methanocaldococcus jannaschii