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Literature summary for 2.7.1.23 extracted from

  • Stephan, C.; Renard, M.; Montrichard, F.
    Evidence for the existence of two soluble NAD(+) kinase isoenzymes in Euglena gracilis Z (2000), Int. J. Biochem. Cell Biol., 32, 855-863.
    View publication on PubMed
Search for more literature for 2.7.1.23

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin independent on calmodulin Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 0.5 mM, 62% inhibition of isoenzyme 1 and 33% inhibition of isoenzyme 2 Euglena gracilis
dithiothreitol 0.1 mM, 81% inhibition, irreversible for isoenzyme 1 and reversible for isoenzyme 2 Euglena gracilis
additional information no inhibition by 2-mercaptoethanol, isoenzyme 1 and 2 Euglena gracilis
PCMB 0.5 mM, 74% inhibition of isoenzyme 1, 23% inhibition of isoenzyme 2 Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 ATP isoenzyme 1 Euglena gracilis
0.2
-
 GTP isoenzyme 2 Euglena gracilis
0.2
-
 GTP GTP in form of MgGTP2- Euglena gracilis
0.26
-
 NAD+ isoenzyme 1 Euglena gracilis
1.03
-
 NAD+ isoenzyme 2 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble isoenzyme 1 and 2 Euglena gracilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Euglena gracilis
-
 isoenzyme 1 and 2
-
Euglena gracilis Z
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + NAD+ = ADP + NADP+ isoenzyme 1: ping-pong mechanism. Isoenzyme 2: sequential mechanism Euglena gracilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+ isoenzyme 1: most effective phosphate donor. Isoenzyme 2: 35% of the activity with GTP Euglena gracilis ADP + NADP+
-
 ?
ATP + NAD+ isoenzyme 1: most effective phosphate donor. Isoenzyme 2: 35% of the activity with GTP Euglena gracilis Z ADP + NADP+
-
 ?
CTP + NAD+ isoenzyme 1: 55% of the activity with ATP. Isoenzyme 2: 77% of the activity with ATP Euglena gracilis CDP + NADP+
-
 ?
CTP + NAD+ isoenzyme 1: 55% of the activity with ATP. Isoenzyme 2: 77% of the activity with ATP Euglena gracilis Z CDP + NADP+
-
 ?
GTP + NAD+
-
 Euglena gracilis GDP + NADP+
-
 ?
GTP + NAD+ isoenzyme 1: 96% of the activity with ATP. Isoenzyme 2: most effective phosphoryl donor Euglena gracilis GDP + NADP+
-
 ?
GTP + NAD+
-
 Euglena gracilis Z GDP + NADP+
-
 ?
GTP + NAD+ isoenzyme 1: 96% of the activity with ATP. Isoenzyme 2: most effective phosphoryl donor Euglena gracilis Z GDP + NADP+
-
 ?
UTP + NAD+ isoenzyme 1: 12% of the activity with ATP. Isoenzyme 2: 19% of the activity with ATP Euglena gracilis UDP + NADP+
-
 ?
UTP + NAD+ isoenzyme 1: 12% of the activity with ATP. Isoenzyme 2: 19% of the activity with ATP Euglena gracilis Z UDP + NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Euglena gracilis