Literature summary for 2.7.1.227 extracted from

Aeed, P.A.; Sperry, A.E.; Young, C.L.; Nagiec, M.M.; Elhammer, A.P.
Effect of membrane perturbants on the activity and phase distribution of inositol phosphorylceramide synthase; development of a novel assay (2004), Biochemistry, 43, 8483-8493 .

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Application

Application	Comment	Organism
analysis	assays using CHAPS- or taurodeoxycholate-washed membranes are reproducible, completely dependent on added acceptor substrate C6-7-nitro-2-1,3-benzoxadiazol-4-yl-ceramide, and more than 95% dependent on added donor substrate phosphatidylinositol. Product formation is linear with respect to both enzyme concentration and time, and transfer efficiency is improved more than 20fold as compared to assays using crude membranes	Candida albicans

Inhibitors

Inhibitors	Comment	Organism
1-phosphatidyl-1D-myo-inositol	substrate inhibition	Candida albicans
amidosulfobetaine-16	inactivates the enzyme irreversibly	Candida albicans
Brij	inactivates the enzyme	Candida albicans
Empigen BB	inactivates the enzyme irreversibly	Candida albicans
N-dodecyl-N,N-(dimethylammonio)butanoate	inactivates the enzyme irreversibly	Candida albicans
Nonidet P-40	inactivates the enzyme	Candida albicans
octylglucoside	inactivates the enzyme	Candida albicans
Triton X-100	inactivates the enzyme	Candida albicans
Tween 20	inactivates the enzyme	Candida albicans
Tween 80	inactivates the enzyme	Candida albicans
Zwittergent 3-10	inactivates the enzyme irreversibly	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0033	-	6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine	CHAPS-washed membranes, pH 7.5, 23°C	Candida albicans
0.138	-	1-phosphatidyl-1D-myo-inositol	CHAPS-washed membranes, pH 7.5, 23°C	Candida albicans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Candida albicans	16020	-

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	O13332	-	-
Candida albicans ATCC MYA-2876	O13332	-	-

Purification (Commentary)

Purification (Comment)	Organism
detergents Triton X-100, Nonidet P-40, Brij, Tween20, Tween 80 and octylglucoside all inactivate the enzyme. At moderate concentrations, the activity of the Triton X-100- and octylglucoside-solubilized material can be partially restored by inclusion of 5 mM phosphatidylinositol in the solubilization buffer. Detergents Empigen BB, N-dodecyl-N,N-(dimethylammonio)butanoate, Zwittergent 3-10, and amidosulfobetaine-16 all inactivate the enzyme essentially irreversibly. IPC synthase activity in membranes remaines largely intact and sedimentable at CHAPS concentrations of 4% where more than 90% of the phospholipids and 60% of the total proteins are extracted from the membranes	Candida albicans

Purification (Comment)

Organism

detergents Triton X-100, Nonidet P-40, Brij, Tween20, Tween 80 and octylglucoside all inactivate the enzyme. At moderate concentrations, the activity of the Triton X-100- and octylglucoside-solubilized material can be partially restored by inclusion of 5 mM phosphatidylinositol in the solubilization buffer. Detergents Empigen BB, N-dodecyl-N,N-(dimethylammonio)butanoate, Zwittergent 3-10, and amidosulfobetaine-16 all inactivate the enzyme essentially irreversibly. IPC synthase activity in membranes remaines largely intact and sedimentable at CHAPS concentrations of 4% where more than 90% of the phospholipids and 60% of the total proteins are extracted from the membranes

Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-phosphatidyl-1D-myo-inositol + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine	-	Candida albicans	?	-	?
1-phosphatidyl-1D-myo-inositol + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine	-	Candida albicans ATCC MYA-2876	?	-	?

Synonyms

Synonyms	Comment	Organism
AUR1	-	Candida albicans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.588	-	CHAPS-washed membranes, pH 7.5, 23°C	Candida albicans	1-phosphatidyl-1D-myo-inositol