Literature summary for 2.7.1.227 extracted from

  • Bromley, P.; Li, Y.; Murphy, S.; Sumner, C.; Lynch, D.
    Complex sphingolipid synthesis in plants Characterization of inositolphosphorylceramide synthase activity in bean microsomes (2003), Arch. Biochem. Biophys., 417, 219-226 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
aureobasidin A
-
Phaseolus vulgaris
CHAPS 0.1% w/v, 44% loss of activity Phaseolus vulgaris
Mn2+ 5 mM, 80% loss of activity Phaseolus vulgaris
additional information neither activating nor inhibitory: 1mM ATP or GTP, 5 mM Ca2+ or Mg2+, 100 mM KCl Phaseolus vulgaris
octylglucoside 0.1% w/v, 40% loss of activity Phaseolus vulgaris
rustmicin
-
Phaseolus vulgaris
taurocholic acid 0.1% w/v, 70% loss of activity Phaseolus vulgaris
Triton X-100 0.1% w/v, 75% loss of activity Phaseolus vulgaris
Tween 20 0.1% w/v, 55% loss of activity Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
1-phosphatidyl-1D-myo-inositol pH 8.0, 30°C Phaseolus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Phaseolus vulgaris
-
-

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Phaseolus vulgaris
-
leaf and hypocotyl, high expression levels Phaseolus vulgaris
-
hypocotyl and leaf, high expression levels Phaseolus vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information maximum product formation is observed at 1-phosphatidyl-1D-myo-inositol concentrations in excess of 600 microM Phaseolus vulgaris ?
-
?
1-phosphatidyl-1D-myo-inositol + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine
-
Phaseolus vulgaris ?
-
?
1-phosphatidyl-1D-myo-inositol + hydroxy fatty acid-containing ceramide
-
Phaseolus vulgaris hydroxy fatty acid-containing inositolphosphoylceramide
-
?
1-phosphatidyl-1D-myo-inositol + nonhydroxy fatty acid-containing ceramide
-
Phaseolus vulgaris nonhydroxy fatty acid-containing inositolphosphoylceramide
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
Phaseolus vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
negligible activity above Phaseolus vulgaris
15
-
negligible activity below Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Phaseolus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
6.6 9.1 at least 50% of mwaximum activity Phaseolus vulgaris

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.4 0.8 pH 8.0, 30°C Phaseolus vulgaris aureobasidin A
16 20 pH 8.0, 30°C Phaseolus vulgaris rustmicin