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Literature summary for 2.7.1.227 extracted from
- Complex sphingolipid synthesis in plants Characterization of inositolphosphorylceramide synthase activity in bean microsomes (2003), Arch. Biochem. Biophys., 417, 219-226 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aureobasidin A | - |
Phaseolus vulgaris |  |
| CHAPS | 0.1% w/v, 44% loss of activity | Phaseolus vulgaris | |
| Mn2+ | 5 mM, 80% loss of activity | Phaseolus vulgaris | |
| additional information | neither activating nor inhibitory: 1mM ATP or GTP, 5 mM Ca2+ or Mg2+, 100 mM KCl | Phaseolus vulgaris | |
| octylglucoside | 0.1% w/v, 40% loss of activity | Phaseolus vulgaris | |
| rustmicin | - |
Phaseolus vulgaris | |
| taurocholic acid | 0.1% w/v, 70% loss of activity | Phaseolus vulgaris | |
| Triton X-100 | 0.1% w/v, 75% loss of activity | Phaseolus vulgaris | |
| Tween 20 | 0.1% w/v, 55% loss of activity | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
1-phosphatidyl-1D-myo-inositol | pH 8.0, 30°C | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Phaseolus vulgaris | 5794 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypocotyl | and leaf, high expression levels | Phaseolus vulgaris | - |
| leaf | and hypocotyl, high expression levels | Phaseolus vulgaris | - |
| seed | - |
Phaseolus vulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine | - |
Phaseolus vulgaris | ? | - |
? | |
| 1-phosphatidyl-1D-myo-inositol + hydroxy fatty acid-containing ceramide | - |
Phaseolus vulgaris | hydroxy fatty acid-containing inositolphosphoylceramide | - |
? | |
| 1-phosphatidyl-1D-myo-inositol + nonhydroxy fatty acid-containing ceramide | - |
Phaseolus vulgaris | nonhydroxy fatty acid-containing inositolphosphoylceramide | - |
? | |
| additional information | maximum product formation is observed at 1-phosphatidyl-1D-myo-inositol concentrations in excess of 600 microM | Phaseolus vulgaris | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Phaseolus vulgaris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
negligible activity below | Phaseolus vulgaris |
| 40 | - |
negligible activity above | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Phaseolus vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 9.1 | at least 50% of mwaximum activity | Phaseolus vulgaris |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.4 | 0.8 | pH 8.0, 30°C | Phaseolus vulgaris | aureobasidin A | |
| 16 | 20 | pH 8.0, 30°C | Phaseolus vulgaris | rustmicin | |
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