Cloned (Comment) | Organism |
---|---|
gene nadR, expression of N-terminally His6-tagged enzyme in Escherichia coli | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | analysis of mutant strains for activities/functions of enzyme domains, reduced activity of class I mutants, overview | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 69% inhibition at 1.2 mM, complete inhibition at 5 mM | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
N-ribosylnicotinamide | recombinant enzyme, pH 7.6, 37°C | Salmonella enterica | |
1.2 | - |
ATP | recombinant enzyme, pH 7.6, 37°C | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-ribosylnicotinamide | Salmonella enterica | activity is essential for N-ribosylnicotinamide assimilation | ADP + nicotinamide ribonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
diverse strains with different genotypes, gene nadR | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Salmonella enterica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide | bifunctional enzyme exhibiting nicotinamide ribose kinase activity, EC 2.7.1.22, and weak adenylyltransferase activity, EC 2.7.7.1 | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
purified recombinant enzyme | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-ribosylnicotinamide | - |
Salmonella enterica | ADP + nicotinamide ribonucleotide | - |
? | |
ATP + N-ribosylnicotinamide | activity is essential for N-ribosylnicotinamide assimilation | Salmonella enterica | ADP + nicotinamide ribonucleotide | - |
? | |
additional information | the enzymes' C-terminal domain has nicotinamide ribose kinase activity, the central domain performes adenylyltransferase reaction, EC 2.7.7.1, overview, the purified enzyme binds nadB promotor sequence in absence of ATP with or without NAD+, DNA binding activity of the enzyme is regulated by ATP binding to the adenylyltransferase domain | Salmonella enterica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NadR | - |
Salmonella enterica |
nicotinamide ribose kinase | - |
Salmonella enterica |
NmR-K | - |
Salmonella enterica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Salmonella enterica |