Cloned (Comment) | Organism |
---|---|
- |
Herpes simplex virus |
Protein Variants | Comment | Organism |
---|---|---|
C336Y | acyclovir resistant, mutation affects ATP binding site | Herpes simplex virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-azido-2'-deoxyuridine | 50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM | Herpes simplex virus | |
5-azido-2'-deoxyuridine-5'-monophosphate | 50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant at 0.135 mM | Herpes simplex virus | |
5-bromovinyldeoxyuridine | 50% photoincorporation inhibition of wild-type enzyme at 0.0015 mM, 50% photoincorporation inhibition of C336Y mutant above 0.025 mM | Herpes simplex virus | |
acyclovir | 50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant above 0.3 mM | Herpes simplex virus | |
ATP | 50% photoincorporation inhibition of wild-type enzyme at 0.15 mM, 50% photoincorporation inhibition of C336Y mutant above 1 mM | Herpes simplex virus | |
thymidine | 50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM | Herpes simplex virus | |
thymidylate | 50% photoincorporation inhibition of wild-type and C336Y mutant enzyme at 0.0125 mM | Herpes simplex virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
thymidine | pH 7.6, 37°C, wild-type | Herpes simplex virus | |
0.0013 | - |
ATP | pH 7.6, 37°C, mutant C336Y | Herpes simplex virus | |
0.01 | - |
thymidine | pH 7.6, 37°C, mutant C336Y | Herpes simplex virus | |
0.03 | - |
ATP | pH 7.6, 37°C, wild-type | Herpes simplex virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herpes simplex virus | - |
type 1 | - |
Purification (Comment) | Organism |
---|---|
- |
Herpes simplex virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.089 | - |
- |
Herpes simplex virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | - |
Herpes simplex virus | ADP + dTMP | - |
? |