Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.21 extracted from

  • Rechtin, T.M.; Black, M.E.; Mao, F.; Lewis, M.L.; Drake, R.R.
    Purification and photoaffinity labeling of herpes simplex virus type-1 thymidine kinase (1995), J. Biol. Chem., 270, 7055-7060.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Herpes simplex virus

Protein Variants

Protein Variants Comment Organism
C336Y acyclovir resistant, mutation affects ATP binding site Herpes simplex virus

Inhibitors

Inhibitors Comment Organism Structure
5-azido-2'-deoxyuridine 50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM Herpes simplex virus
5-azido-2'-deoxyuridine-5'-monophosphate 50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant at 0.135 mM Herpes simplex virus
5-bromovinyldeoxyuridine 50% photoincorporation inhibition of wild-type enzyme at 0.0015 mM, 50% photoincorporation inhibition of C336Y mutant above 0.025 mM Herpes simplex virus
acyclovir 50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant above 0.3 mM Herpes simplex virus
ATP 50% photoincorporation inhibition of wild-type enzyme at 0.15 mM, 50% photoincorporation inhibition of C336Y mutant above 1 mM Herpes simplex virus
thymidine 50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM Herpes simplex virus
thymidylate 50% photoincorporation inhibition of wild-type and C336Y mutant enzyme at 0.0125 mM Herpes simplex virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
thymidine pH 7.6, 37°C, wild-type Herpes simplex virus
0.0013
-
ATP pH 7.6, 37°C, mutant C336Y Herpes simplex virus
0.01
-
thymidine pH 7.6, 37°C, mutant C336Y Herpes simplex virus
0.03
-
ATP pH 7.6, 37°C, wild-type Herpes simplex virus

Organism

Organism UniProt Comment Textmining
Herpes simplex virus
-
type 1
-

Purification (Commentary)

Purification (Comment) Organism
-
Herpes simplex virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.089
-
-
Herpes simplex virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine
-
Herpes simplex virus ADP + dTMP
-
?