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Literature summary for 2.7.1.21 extracted from

  • Ellims, P.H.; van der Weyden, M.B.
    Human liver thymidine kinase. Purification and some properties of the enzyme (1980), J. Biol. Chem., 255, 11290-11295.
    View publication on PubMed

General Stability

General Stability Organism
not stabilized by 10% glycerol, 5 mM 2-mercaptoethanol, 5 mM EDTA, 1 mg/ml bovine serum albumin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Homo sapiens
49000
-
gel filtration Homo sapiens
340000
-
oligomeric form, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, or 4°C, 24 h, more than 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine
-
Homo sapiens ADP + dTMP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
enzyme TK-A: half-life 8 h, enzyme TK-C: half-life 30 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
bimodal pH profile with a peak at pH 7.4 and a minor peak at pH 9.0 Homo sapiens
9
-
bimodal pH profile with a peak at pH 7.4 and a minor peak at pH 9.0 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens linear pH gradient
-
5.1