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Literature summary for 2.7.1.21 extracted from

  • Gan, T.E.; Brumley, J.L.; van der Weyden, M.B.
    Human thymidine kinase. Purification and properties of the cytosolic enzyme of placenta (1983), J. Biol. Chem., 258, 7000-7004.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
thymidine pH 7.4 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially substitute for Mg2+ Homo sapiens
Mg2+
-
Homo sapiens
Mn2+ can partially substitute for Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 * 44000, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
84000 98000
-
Homo sapiens
92000
-
gel filtration, sucrose density gradient centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.031
-
liver enzyme Homo sapiens
0.094
-
placenta enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-bromo-2'-deoxyuridine
-
Homo sapiens ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
?
ATP + 5-diaza-2'-deoxyuridine
-
Homo sapiens ADP + 5-diaza-2'-deoxyuridine 5'-phosphate
-
?
ATP + 5-iodo-2'-deoxyuridine
-
Homo sapiens ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
?
ATP + thymidine ATP can be substituted by GTP Homo sapiens ADP + dTMP
-
?
ATP + thymidine ATP can be substituted by dGTP Homo sapiens ADP + dTMP
-
?
ATP + thymidine ATP can be substituted by dCTP Homo sapiens ADP + dTMP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens linear pH gradient between pH 7 and pH 11
-
9.1