Literature summary for 2.7.1.207 extracted from

Sossna-Wunder, G.; Hengstenberg, W.; Briozzo, P.; Deutscher, J.
Photoinactivation of the Staphylococcus aureus lactose-specific EIICB phosphotransferase component with p-azidophenyl-beta-D-galactoside and phosphorylation of the covalently bound substrate (2018), J. Mol. Microbiol. Biotechnol., 28, 147-158 .

Search for more literature for 2.7.1.207

Cloned(Commentary)

Cloned (Comment)	Organism
-	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
4-azidophenyl-beta-D-galactoside	irradiation of a reaction mixture containing 0.25 mM of photoactivatable 4-azidophenyl-beta-D-galactopyranoside and enzyme with UV light at 254 nm for 3 min causes approximately 90% loss of enzyme activity. When the photoinactivation experiments are carried out in the presence of 1 mM lactose, the loss of enzyme activity is significantly slowed	Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Staphylococcus aureus	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1]	Staphylococcus aureus	-	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?
[protein]-Npi-phospho-L-histidine + lactose[side 1]	Staphylococcus aureus S305A	-	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus S305A	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzyme's EIIB domain is phosphorylated at Cys476	Staphylococcus aureus

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus

Storage Stability

Storage Stability	Organism
-80Â°C, purified enzyme, several months, no significant loss of activity	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	4-nitrophenyl-alpha-D-galactopyranoside is a poor substrate for the enzyme	Staphylococcus aureus	?	-	-
additional information	4-nitrophenyl-alpha-D-galactopyranoside is a poor substrate for the enzyme	Staphylococcus aureus S305A	?	-	-
[protein]-Npi-phospho-L-histidine + 4-nitrophenyl beta-D-galactoside[side 1]	-	Staphylococcus aureus	[protein]-L-histidine + 4-nitrophenyl beta-D-galactoside 6'-phosphate[side 2]	-	?
[protein]-Npi-phospho-L-histidine + 4-nitrophenyl beta-D-galactoside[side 1]	-	Staphylococcus aureus S305A	[protein]-L-histidine + 4-nitrophenyl beta-D-galactoside 6'-phosphate[side 2]	-	?
[protein]-Npi-phospho-L-histidine + lactose[side 1]	-	Staphylococcus aureus	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?
[protein]-Npi-phospho-L-histidine + lactose[side 1]	-	Staphylococcus aureus S305A	[protein]-L-histidine + lactose 6'-phosphate[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE	Staphylococcus aureus
?	x * 62700, calculated from amino acid sequence	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
EIICBLac	-	Staphylococcus aureus
lactose-specific permease enzyme IICB	-	Staphylococcus aureus

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Release 2023.1 (January 2023)