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Literature summary for 2.7.1.207 extracted from

  • Kowolik, C.; Hengstenberg, W.
    The lactose transporter of Staphylococcus aureus - Overexpression, purification and characterization of the histidine-tagged domains IIC and IIB (1998), Eur. J. Biochem., 257, 389-394.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
domain IIC fused with a C-terminal affinity tag of six histidine residues and domain IIB fused with a N-terminal histidine tag are expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
C476S inactive IICBlac mutant Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
2-(N-dansyl)aminoethyl alpha-D-thiogalactoside
-
Staphylococcus aureus
isopropyl thio-beta-D-galactoside
-
Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Staphylococcus aureus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1] Staphylococcus aureus
-
[protein]-L-histidine + lactose 6'-phosphate[side 2]
-
?
[protein]-Npi-phospho-L-histidine + lactose[side 1] Staphylococcus aureus 825A
-
[protein]-L-histidine + lactose 6'-phosphate[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus 825A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni21 chelate affinity column chromatography Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0008
-
unpurified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C Staphylococcus aureus
0.0433
-
54.1fold purified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[protein]-Npi-phospho-L-histidine + lactose[side 1]
-
Staphylococcus aureus [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
?
[protein]-Npi-phospho-L-histidine + lactose[side 1]
-
Staphylococcus aureus 825A [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
?

Synonyms

Synonyms Comment Organism
IICBlac
-
Staphylococcus aureus
lactose transporter
-
Staphylococcus aureus
lactose-specific enzyme II
-
Staphylococcus aureus