Cloned (Comment) | Organism |
---|---|
domain IIC fused with a C-terminal affinity tag of six histidine residues and domain IIB fused with a N-terminal histidine tag are expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
C476S | inactive IICBlac mutant | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(N-dansyl)aminoethyl alpha-D-thiogalactoside | - |
Staphylococcus aureus | |
isopropyl thio-beta-D-galactoside | - |
Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Staphylococcus aureus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-Npi-phospho-L-histidine + lactose[side 1] | Staphylococcus aureus | - |
[protein]-L-histidine + lactose 6'-phosphate[side 2] | - |
? | |
[protein]-Npi-phospho-L-histidine + lactose[side 1] | Staphylococcus aureus 825A | - |
[protein]-L-histidine + lactose 6'-phosphate[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus 825A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni21 chelate affinity column chromatography | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0008 | - |
unpurified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C | Staphylococcus aureus |
0.0433 | - |
54.1fold purified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-Npi-phospho-L-histidine + lactose[side 1] | - |
Staphylococcus aureus | [protein]-L-histidine + lactose 6'-phosphate[side 2] | - |
? | |
[protein]-Npi-phospho-L-histidine + lactose[side 1] | - |
Staphylococcus aureus 825A | [protein]-L-histidine + lactose 6'-phosphate[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IICBlac | - |
Staphylococcus aureus |
lactose transporter | - |
Staphylococcus aureus |
lactose-specific enzyme II | - |
Staphylococcus aureus |