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Literature summary for 2.7.1.20 extracted from

  • Park, J.; Singh, B.; Maj, M.C.; Gupta, R.S.
    Phosphorylated derivatives that activate or inhibit mammalian adenosine kinase provide insights into the role of pentavalent ions in AK catalysis (2004), Protein J., 23, 167-177.
    View publication on PubMed
Search for more literature for 2.7.1.20

Activating Compound

Activating Compound Comment Organism Structure
creatine phosphate 8fold increase in Vmax at 20 mM Cricetulus griseus
additional information no significant stimulation by O-phosphorylethanolamine, glucose 6-phosphate, D-3-phosphoglyceric acid, phospho-L-serine Cricetulus griseus
phosphate antagonized by phosphonoacetic acid Cricetulus griseus
phosphoenolpyruvate 5fold increase in Vmax at 20 mM Cricetulus griseus
Phosphonoformic acid
-
 Cricetulus griseus
phosphoribosyl diphosphate 5fold increase in Vmax at 20 mM Cricetulus griseus
Sodium diphosphate
-
 Cricetulus griseus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cricetulus griseus

Inhibitors

Inhibitors Comment Organism Structure
2-carboxyethyl phosphonic acid
-
 Cricetulus griseus
N-phosphomethyl glycine
-
 Cricetulus griseus
N-phosphomethyl iminodiacetic acid
-
 Cricetulus griseus
Phosphonoacetic acid 64% inhibition at 8 mM, antagonized by phosphate Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 1-10 mM phosphate Cricetulus griseus
0.0017
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 10-20 mM creatine phosphate Cricetulus griseus
0.0022
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 20 mM phosphoribosyl diphosphate Cricetulus griseus
0.0023
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 20 mM phosphoenolpyruvate Cricetulus griseus
0.0075
-
 adenosine recombinant enzyme, pH 7.2, 37°C Cricetulus griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine Cricetulus griseus purine salvage enzyme ADP + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Cricetulus griseus

Reaction

Reaction Comment Organism Reaction ID
ATP + adenosine = ADP + AMP catalytic mechanism, substrate and product binding involving Asp239, Glu242, Asp316, and Arg148 Cricetulus griseus
a

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000066
-
 purifed recombinant enzyme Cricetulus griseus
0.000305
-
 purifed recombinant enzyme, in presence of 10 mM phosphoribosyl diphosphate Cricetulus griseus
0.000365
-
 purifed recombinant enzyme, in presence of 10 mM phosphoenolpyruvate Cricetulus griseus
0.000544
-
 purifed recombinant enzyme, in presence of 10 mM creatine phosphate Cricetulus griseus
0.000888
-
 purifed recombinant enzyme, in presence of 10 mM phosphate Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine
-
 Cricetulus griseus ADP + AMP
-
 ?
ATP + adenosine purine salvage enzyme Cricetulus griseus ADP + AMP
-
 ?

Synonyms

Synonyms Comment Organism
AK
-
 Cricetulus griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cricetulus griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 adenosine recombinant enzyme, pH 7.2, 37°C Cricetulus griseus
0.33
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 20 mM phosphoribosyl diphosphate Cricetulus griseus
0.4
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 20 mM phosphoenolpyruvate Cricetulus griseus
0.59
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 20 mM creatine phosphate Cricetulus griseus
0.97
-
 adenosine recombinant enzyme, pH 7.2, 37°C, in presence of 10 mM phosphate Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Cricetulus griseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.95
-
 Phosphonoacetic acid recombinant enzyme, pH 7.2, 37°C Cricetulus griseus