Any feedback?
Literature summary for 2.7.1.20 extracted from
- Identification of four adenosine kinase isoforms in tobacco By-2 cells and their putative role in the cell cycle-regulated cytokinin metabolism (2005), J. Biol. Chem., 280, 17512-17519.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis of the ADK protein family, DNA and amino acid sequence determination and analysis, expression of His-tagged protein in Escherichia coli strain BL21(DE3) | Nicotiana tabacum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | at 1 mM MgCl2, ATP:Mg2+ ratio of 4:1 | Nicotiana tabacum |  |
| NaF | at 30 mM | Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, about, recombinant enzyme, SDS-PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | Nicotiana tabacum | key enzyme in regulation of intracellular adenosine level, responsible, especially isozyme ADK2S, for the conversion of cytokinin ribosides into their respective nucleotides, accumulation of enzyme and increased activity just prior to mitosis is associated with a very active cytokinin metabolism at that phase of the cell cycle in cell culture, overview | ADP + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q5DKU6 | isozyme 2T; 4 isozymes 1S, 2S, 1T, and 2T | - |
| Nicotiana tabacum | Q5DKU8 | isozyme 2S; 4 isozymes 1S, 2S, 1T, and 2T | - |
| Nicotiana tabacum | Q5DKU9 | isozyme 1S; 4 isozymes 1S, 2S, 1T, and 2T | - |
| Nicotiana tabacum | Q5DKV0 | isozyme 1T; 4 isozymes 1S, 2S, 1T, and 2T | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Nicotiana tabacum | - |
| additional information | analysis of modulation of the expression patterns of isozymes ADK1S, ADK1T, ADK2S, and ADK2T during the cell cycle, overview | Nicotiana tabacum | - |
| TBY-2 cell | - |
Nicotiana tabacum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | - |
Nicotiana tabacum | ADP + AMP | - |
? | |
| ATP + adenosine | key enzyme in regulation of intracellular adenosine level, responsible, especially isozyme ADK2S, for the conversion of cytokinin ribosides into their respective nucleotides, accumulation of enzyme and increased activity just prior to mitosis is associated with a very active cytokinin metabolism at that phase of the cell cycle in cell culture, overview | Nicotiana tabacum | ADP + AMP | - |
? | |
| ATP + adenosine | ATP:Mg2+ ratio of 4:1 | Nicotiana tabacum | ADP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, about, recombinant enzyme, SDS-PAGE | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADK | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | ATP:Mg2+ ratio of 4:1 | Nicotiana tabacum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
