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Literature summary for 2.7.1.20 extracted from
- Identification and characterization of a unique adenosine kinase from Mycobacterium tuberculosis (2003), J. Bacteriol., 185, 6548-6555.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | enzyme is a target for drug development in tuberculosis treatment of humans due to the structural and substrate specificity differences of human and mycobacterial enzymes, overview | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Rv2202c or adoK, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in enzyme-deficient Mycobacterium smegmatis strain SRI101 and in enzyme-deficient Mycobacterium tuberculosis strain SRICKI | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | substrate inhibition above 0.005 mM, 25% inhibition at 0.025 mM | Mycobacterium tuberculosis |  |
| iodotubercidin | competitive | Mycobacterium tuberculosis | |
| Mg2+ | dependent on, optimal at 10-50 mM MgCl2, inhibitory above 50 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
adenosine | native enzyme, pH 8.0, 37°C, in presence of 10 mM KCl | Mycobacterium tuberculosis | |
| 0.0034 | - |
adenosine | native enzyme, pH 8.0, 37°C, in absence of KCl | Mycobacterium tuberculosis | |
| 0.079 | - |
2-methyladenosine | native enzyme, pH 8.0, 37°C, in presence of 10 mM KCl | Mycobacterium tuberculosis | |
| 0.709 | - |
2-methyladenosine | native enzyme, pH 8.0, 37°C, in absence of KCl | Mycobacterium tuberculosis | |
| 1.1 | - |
ATP | native enzyme, pH 8.0, 37°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulates | Mycobacterium tuberculosis | |
| Mg2+ | dependent on, optimal at 10-50 mM MgCl2, inhibitory above 50 mM | Mycobacterium tuberculosis | |
| additional information | no stimulation by Na+ or Li+, or by phosphate | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34337 | - |
2 * 35000, native enzyme, SDS-PAGE, 2 * 34337, native enzyme, mass spectrometry | Mycobacterium tuberculosis |
| 35000 | - |
2 * 35000, native enzyme, SDS-PAGE, 2 * 34337, native enzyme, mass spectrometry | Mycobacterium tuberculosis |
| 70000 | - |
about, native enzyme, sedimentation equilibrium analysis | Mycobacterium tuberculosis |
| 74840 | - |
native enzyme, light scattering analysis | Mycobacterium tuberculosis |
| 136000 | - |
native enzyme, gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-methyladenosine | Mycobacterium tuberculosis | activation of 2-mehyladenosine | ADP + 2-methyl-AMP | - |
? | |
| ATP + 2-methyladenosine | Mycobacterium tuberculosis H37Ra / ATCC 25177 | activation of 2-mehyladenosine | ADP + 2-methyl-AMP | - |
? | |
| ATP + adenosine | Mycobacterium tuberculosis | purine salvage enzyme | ADP + AMP | - |
? | |
| ATP + adenosine | Mycobacterium tuberculosis H37Ra / ATCC 25177 | purine salvage enzyme | ADP + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | A5U4N0 | gene Rv2202c or adoK; gene Rv2202c or adoK | - |
| Mycobacterium tuberculosis H37Ra / ATCC 25177 | A5U4N0 | gene Rv2202c or adoK; gene Rv2202c or adoK | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 100fold to homogeneity from strain H37Ra | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.005 | - |
wild-type strain H37Ra | Mycobacterium tuberculosis |
| 0.1 | - |
enzyme in recombinant strain SRICKI | Mycobacterium tuberculosis |
| 0.36 | - |
enzyme in recombinant Mycobacterium smegmatis strain SRI101 | Mycobacterium tuberculosis |
| 0.39 | - |
purified native enzyme | Mycobacterium tuberculosis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified native enzyme, 20% glycerol, stable up to 6 months | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-methyladenosine | activation of 2-mehyladenosine | Mycobacterium tuberculosis | ADP + 2-methyl-AMP | - |
? | |
| ATP + 2-methyladenosine | 30fold lower activity compared to adenosine | Mycobacterium tuberculosis | ADP + 2-methyl-AMP | - |
? | |
| ATP + 2-methyladenosine | activation of 2-mehyladenosine | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + 2-methyl-AMP | - |
? | |
| ATP + adenosine | - |
Mycobacterium tuberculosis | ADP + AMP | - |
? | |
| ATP + adenosine | purine salvage enzyme | Mycobacterium tuberculosis | ADP + AMP | - |
? | |
| ATP + adenosine | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + AMP | - |
? | |
| ATP + adenosine | purine salvage enzyme | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + AMP | - |
? | |
| dGTP + adenosine | 4.7fold higher activity compared to ATP | Mycobacterium tuberculosis | dGDP + AMP | - |
? | |
| GTP + adenosine | 2.5fold higher activity compared to ATP | Mycobacterium tuberculosis | GDP + AMP | - |
? | |
| GTP + adenosine | 2.5fold higher activity compared to ATP | Mycobacterium tuberculosis H37Ra / ATCC 25177 | GDP + AMP | - |
? | |
| additional information | dATP, UTP, dTTP, CTP, and dCTP are poor phosphate donors | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | dATP, UTP, dTTP, CTP, and dCTP are poor phosphate donors | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, native enzyme, SDS-PAGE, 2 * 34337, native enzyme, mass spectrometry | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AK | - |
Mycobacterium tuberculosis |
| Rv2202c | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
about | Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | rapid decrease in activity below pH 8.0 and above pH 11.0 | Mycobacterium tuberculosis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00021 | - |
iodotubercidin | pH 8.0, 37°C | Mycobacterium tuberculosis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium tuberculosis | calculation | - |
4.3 |
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