General Stability | Organism |
---|---|
ATP or GTP stabilizes during gel filtration | Trypanosoma cruzi |
does not withstand dialysis against Tris-dithioerythritol buffer, pH 8, with or without 1 mM ATP | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Amino-5-imidazolecarboxamide riboside | - |
Trypanosoma cruzi | |
6-dimethylaminopurine riboside | - |
Trypanosoma cruzi | |
6-mercaptopurine riboside | weak inhibition | Trypanosoma cruzi | |
6-methylaminopurine riboside | - |
Trypanosoma cruzi | |
6-methylmercaptopurine riboside phosphate | - |
Trypanosoma cruzi | |
8-azaguanosine | weak inhibition | Trypanosoma cruzi | |
adenosine | - |
Trypanosoma cruzi | |
ATP | no inhibition | Trypanosoma cruzi | |
deoxyadenosine | - |
Trypanosoma cruzi | |
Deoxycoformycin | - |
Trypanosoma cruzi | |
deoxyguanosine | - |
Trypanosoma cruzi | |
guanosine | weak inhibition | Trypanosoma cruzi | |
Mg2+ | not | Trypanosoma cruzi | |
tubercidin | - |
Trypanosoma cruzi | |
Xanthosine | weak inhibition | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
trypanosomatid flagellate | - |
Storage Stability | Organism |
---|---|
-80°C to -20°C, 6 months | Trypanosoma cruzi |
4°C, with DTT or 2-mercaptoethanol, about 90% loss of activity, ATP or GTP does not stabilize | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | - |
Trypanosoma cruzi | ADP + AMP | - |
? | |
GTP + adenosine | equally effective as ATP | Trypanosoma cruzi | GDP + AMP | - |
? | |
ITP + adenosine | less than with ATP | Trypanosoma cruzi | IDP + AMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Trypanosoma cruzi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heat labile | Trypanosoma cruzi |
55 | - |
1 min, inactivation | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Trypanosoma cruzi |