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Literature summary for 2.7.1.20 extracted from

  • Chang, C.H.; Brockman, R.W.; Bennett, L.L.
    Adenosine kinase from L1210 cells. Purification and some properties of the enzyme (1980), J. Biol. Chem., 255, 2366-2371.
    View publication on PubMed
Search for more literature for 2.7.1.20

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin
-
 Mus musculus
additional information no activation by polyamines Mus musculus

General Stability

General Stability Organism
bovine serum albumin stabilizes Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ above 0.7 mM Mus musculus
additional information no inhibition by polyamines Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 adenosine pH 6.8, 37ºC, 12 mM ATP and 3 mM MgCl2 Mus musculus
0.002
-
 adenosine pH 6.8, 37ºC, 1 mM ATP and 0.7 mM MgCl2 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Mus musculus
Co2+ can partially substitute for Mg2+ Mus musculus
Co2+ activation Mus musculus
Fe2+ activation Mus musculus
Fe2+ sulfate form is more effective than the chloride form Mus musculus
Mg2+ requirement Mus musculus
Mg2+ the chloride form is more effective than the sulfate form Mus musculus
Mn2+ can partially substitute for Mg2+ Mus musculus
Mn2+ activation Mus musculus
Ni2+ can partially substitute for Mg2+ Mus musculus
Ni2+ activation Mus musculus
Zn2+ no activation Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 PAGE, sucrose density gradient centrifugation, gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin sulfate and ammonium sulfate fractionation, chromatography on DEAE-cellulose and affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
 Mus musculus
-
leukemia cell L1210, implanted in mice Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.305
-
-
 Mus musculus

Storage Stability

Storage Stability Organism
-20°C, in 45% sucrose, at least 1 month Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine strictly specific for adenosine Mus musculus ADP + AMP
-
 ?
CTP + adenosine CTP is a poor substrate Mus musculus CDP + AMP
-
 ?
GTP + adenosine best substrate Mus musculus GDP + AMP
-
 ?
ITP + adenosine about half as effective as GTP Mus musculus IDP + AMP
-
 ?
ITP + adenosine about half as effective as ATP Mus musculus IDP + AMP
-
 ?
UTP + adenosine UTP is a poor substrate Mus musculus UDP + AMP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 37 60 min stable at pH 6.8 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the pH-optimum is a function of the ATP/Mg2+ ratio Mus musculus
6.8
-
-
 Mus musculus