Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | - |
Mus musculus | |
additional information | no activation by polyamines | Mus musculus |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | above 0.7 mM | Mus musculus | |
additional information | no inhibition by polyamines | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
adenosine | pH 6.8, 37ºC, 12 mM ATP and 3 mM MgCl2 | Mus musculus | |
0.002 | - |
adenosine | pH 6.8, 37ºC, 1 mM ATP and 0.7 mM MgCl2 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Mus musculus | |
Co2+ | can partially substitute for Mg2+ | Mus musculus | |
Co2+ | activation | Mus musculus | |
Fe2+ | activation | Mus musculus | |
Fe2+ | sulfate form is more effective than the chloride form | Mus musculus | |
Mg2+ | requirement | Mus musculus | |
Mg2+ | the chloride form is more effective than the sulfate form | Mus musculus | |
Mn2+ | can partially substitute for Mg2+ | Mus musculus | |
Mn2+ | activation | Mus musculus | |
Ni2+ | can partially substitute for Mg2+ | Mus musculus | |
Ni2+ | activation | Mus musculus | |
Zn2+ | no activation | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
PAGE, sucrose density gradient centrifugation, gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
streptomycin sulfate and ammonium sulfate fractionation, chromatography on DEAE-cellulose and affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
leukemia cell | L1210, implanted in mice | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.305 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, in 45% sucrose, at least 1 month | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | strictly specific for adenosine | Mus musculus | ADP + AMP | - |
? | |
CTP + adenosine | CTP is a poor substrate | Mus musculus | CDP + AMP | - |
? | |
GTP + adenosine | best substrate | Mus musculus | GDP + AMP | - |
? | |
ITP + adenosine | about half as effective as GTP | Mus musculus | IDP + AMP | - |
? | |
ITP + adenosine | about half as effective as ATP | Mus musculus | IDP + AMP | - |
? | |
UTP + adenosine | UTP is a poor substrate | Mus musculus | UDP + AMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | 60 min stable at pH 6.8 | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the pH-optimum is a function of the ATP/Mg2+ ratio | Mus musculus |
6.8 | - |
- |
Mus musculus |