Cloned (Comment) | Organism |
---|---|
the KLLA0C01155g gene is codon optimized for improved expression in Escherichia coli. Expression in Escherichia coli BL21 (DE3) | Kluyveromyces lactis |
Crystallization (Comment) | Organism |
---|---|
the enzyme is crystallized in an apo form. Sitting-drop vapor diffusion at room temperature | Kluyveromyces lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | the enzyme exists in two states with different catalytic activity, with an apparent transition point at 0.045 mM ATP. At higher ATP concentrations, strong inhibition takes place | Kluyveromyces lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
D-glucose | pH and temperature not specified in the publication | Kluyveromyces lactis | |
0.15 | - |
ATP | pH and temperature not specified in the publication | Kluyveromyces lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | Q6CUZ3 | - |
- |
Kluyveromyces lactis ATCC 8585 | Q6CUZ3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | no activity with fructose | Kluyveromyces lactis | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | no activity with fructose | Kluyveromyces lactis ATCC 8585 | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KlGlk1 | - |
Kluyveromyces lactis |
KLLA0C01155g | - |
Kluyveromyces lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41.9 | - |
unfolding temperature in thermal shift assay | Kluyveromyces lactis |
53.6 | - |
unfolding temperature in Tycho analysis | Kluyveromyces lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
D-glucose | pH and temperature not specified in the publication | Kluyveromyces lactis | |
400 | - |
ATP | pH and temperature not specified in the publication | Kluyveromyces lactis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is an essential component required for glucose signaling | Kluyveromyces lactis |