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Literature summary for 2.7.1.2 extracted from

  • Zak, K.M.; Kalilska, M.; Wator, E.; Kuska, K.; Krutyholowa, R.; Dubin, G.; Popowicz, G.M.; Grudnik, P.
    Crystal structure of Kluyveromyces lactis glucokinase (KlGlk1) (2019), Int. J. Mol. Sci., 20, 4821 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the KLLA0C01155g gene is codon optimized for improved expression in Escherichia coli. Expression in Escherichia coli BL21 (DE3) Kluyveromyces lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
the enzyme is crystallized in an apo form. Sitting-drop vapor diffusion at room temperature Kluyveromyces lactis

Inhibitors

Inhibitors Comment Organism Structure
ATP the enzyme exists in two states with different catalytic activity, with an apparent transition point at 0.045 mM ATP. At higher ATP concentrations, strong inhibition takes place Kluyveromyces lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
D-glucose pH and temperature not specified in the publication Kluyveromyces lactis
0.15
-
ATP pH and temperature not specified in the publication Kluyveromyces lactis

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis Q6CUZ3
-
-
Kluyveromyces lactis ATCC 8585 Q6CUZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose no activity with fructose Kluyveromyces lactis ADP + D-glucose 6-phosphate
-
?
ATP + D-glucose no activity with fructose Kluyveromyces lactis ATCC 8585 ADP + D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
KlGlk1
-
Kluyveromyces lactis
KLLA0C01155g
-
Kluyveromyces lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41.9
-
unfolding temperature in thermal shift assay Kluyveromyces lactis
53.6
-
unfolding temperature in Tycho analysis Kluyveromyces lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
150
-
D-glucose pH and temperature not specified in the publication Kluyveromyces lactis
400
-
ATP pH and temperature not specified in the publication Kluyveromyces lactis

General Information

General Information Comment Organism
metabolism the enzyme is an essential component required for glucose signaling Kluyveromyces lactis