Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is 2.12fold induced by growth on glucose compared to citrate | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene glk is organized in gene clusters, transcriptomic analysis of KT2440 in response to glucose, overview | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-specific homologous inactivation of gene glk to construct a mutant strain, activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, glk mutants exhibit a lower total carbon consumption rate than the parental strain, overview | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Pseudomonas putida | the enzyme initiates the intracellular glucose catabolism/metabolism, overview | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | Pseudomonas putida KT 2240 | the enzyme initiates the intracellular glucose catabolism/metabolism, overview | ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
and isogenic mutant strains, gene glk | - |
Pseudomonas putida KT 2240 | - |
and isogenic mutant strains, gene glk | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, overview | Pseudomonas putida |
0.0018 | - |
strain KT2440, grown on citrate | Pseudomonas putida |
0.0211 | - |
strain KT2440, grown on glucose | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Pseudomonas putida | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | the enzyme initiates the intracellular glucose catabolism/metabolism, overview | Pseudomonas putida | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Pseudomonas putida KT 2240 | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | the enzyme initiates the intracellular glucose catabolism/metabolism, overview | Pseudomonas putida KT 2240 | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glk | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas putida |