Literature summary for 2.7.1.197 extracted from

Elferink, M.; Driessen, A.; Ribillard, G.
Functional reconstitution of the purified phosphoenolpyruvate-dependent mannitol-specific transport system of Escherichia coli in phospholipid vesicles: Coupling between transport and phosphorylation (1990), J. Bacteriol., 172, 7119-7125.

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Inhibitors

Inhibitors	Comment	Organism	Structure
N-ethylmaleimide	92% inhibition at 4 mM	Escherichia coli
Trypsin	0.0005 mg/ml trypsin degrades half of the enzyme molecules in intact liposomes	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	D-mannitol[side 1]	at pH 7.5 and 30°C	Escherichia coli
0.066	-	D-mannitol 1-phosphate[side 2]	at pH 7.5 and 30°C	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]	Escherichia coli	-	[protein]-L-histidine + D-mannitol 1-phosphate[side 2]	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1]	-	Escherichia coli	[protein]-L-histidine + D-mannitol 1-phosphate[side 2]	-	r

Synonyms

Synonyms	Comment	Organism
EIIMtl	-	Escherichia coli
mannitol-specific enzyme II	-	Escherichia coli

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Release 2023.1 (January 2023)