Literature summary for 2.7.1.197 extracted from

Van Montfort, R.L.M.; Pijning, T.; Kalk, K.H.; Hangyi, I.; Kouwijzer, M.L.C.E.; Robillard, G.T.; Dijkstra, B.W.
The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site (1998), Structure, 6, 377-388.

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Cloned(Commentary)

Cloned (Comment)	Organism
selenomethionine-labelled enzyme is expressed in Escherichia coli LE392 cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
mannitol-specific IIA domain in complex with HPr protein, vapor diffusion method, using 36-38% (w/v) PEG4000, 0.1 mM N-Tris(hydroxymethyl)methyl-3-aminopropane sulfonic acid/NaOH, pH 8.7, 5 mM Na2EDTA and 1 mM NaN3	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
16300	-	x * 16300, mannitol-specific IIA domain, multiple anomalous dispersion analysis	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1]	Escherichia coli	-	[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00550	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1]	-	Escherichia coli	[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 16300, mannitol-specific IIA domain, multiple anomalous dispersion analysis	Escherichia coli

Synonyms

Synonyms	Comment	Organism
IIAmtl	enzyme domain	Escherichia coli
mannitol-specific EII	-	Escherichia coli
phosphotransferase IIAmannitol	-	Escherichia coli

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Release 2023.1 (January 2023)