BRENDA - Enzyme Database
show all sequences of 2.7.1.196

Analytical sedimentation of the IIA(Chb) and IIB(Chb) proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex

Keyhani, N.; Rodgers, M.; Demeler, B.; Hansen, J.; Roseman, S.; J. Biol. Chem. 275, 33110-33115 (2000)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
10480
-
subunit IIBChb, sedimentation equilibrium analysis
Escherichia coli
11431
-
1 * 11431, subunit IIBChb, calculated from amino acid sequence
Escherichia coli
12748
-
2 * 12748, subunit IIAChb, calculated from amino acid sequence
Escherichia coli
25200
-
subunit IIAChb, sedimentation equilibrium analysis
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[protein]-Npi-phospho-L-histidine + N,N'-diacetylchitobiose[side 1]
Escherichia coli
-
[protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate[side 2]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[protein]-Npi-phospho-L-histidine + N,N'-diacetylchitobiose[side 1]
-
736398
Escherichia coli
[protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate[side 2]
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 12748, subunit IIAChb, calculated from amino acid sequence
Escherichia coli
monomer
1 * 11431, subunit IIBChb, calculated from amino acid sequence
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
10480
-
subunit IIBChb, sedimentation equilibrium analysis
Escherichia coli
11431
-
1 * 11431, subunit IIBChb, calculated from amino acid sequence
Escherichia coli
12748
-
2 * 12748, subunit IIAChb, calculated from amino acid sequence
Escherichia coli
25200
-
subunit IIAChb, sedimentation equilibrium analysis
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[protein]-Npi-phospho-L-histidine + N,N'-diacetylchitobiose[side 1]
Escherichia coli
-
[protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate[side 2]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[protein]-Npi-phospho-L-histidine + N,N'-diacetylchitobiose[side 1]
-
736398
Escherichia coli
[protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate[side 2]
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 12748, subunit IIAChb, calculated from amino acid sequence
Escherichia coli
monomer
1 * 11431, subunit IIBChb, calculated from amino acid sequence
Escherichia coli
Other publictions for EC 2.7.1.196
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736346
Uchiyama
Uptake of N,N-diacetylchitobio ...
Serratia marcescens, Serratia marcescens 2170
J. Bacteriol.
185
1776-1782
2003
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736398
Keyhani
Analytical sedimentation of th ...
Escherichia coli
J. Biol. Chem.
275
33110-33115
2000
-
-
-
-
-
-
-
-
-
-
4
1
-
2
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737219
Reizer
The cellobiose permease of Esc ...
Escherichia coli
Res. Microbiol.
141
1061-1067
1990
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-