BRENDA - Enzyme Database show
show all sequences of 2.7.1.195

Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor

Sampaio, M.; Chevance, F.; Dippel, R.; Eppler, T.; Schlegel, A.; Boos, W.; Lu, Y.; Rock, C.; J. Biol. Chem. 279, 5537-5548 (2004)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
2-O-alpha-mannosyl-D-glycerate [side 1]
apparent value, at pH 7.6, temperature not specified in the publication
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[protein]-Npi-phospho-L-histidine + 2-O-alpha-mannosyl-D-glycerate [side 1]
Escherichia coli
-
[protein]-L-histidine + 2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate [side 2]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P54745
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[protein]-Npi-phospho-L-histidine + 2-O-alpha-mannosyl-D-glycerate [side 1]
-
715472
Escherichia coli
[protein]-L-histidine + 2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate [side 2]
-
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
2-O-alpha-mannosyl-D-glycerate [side 1]
apparent value, at pH 7.6, temperature not specified in the publication
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[protein]-Npi-phospho-L-histidine + 2-O-alpha-mannosyl-D-glycerate [side 1]
Escherichia coli
-
[protein]-L-histidine + 2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate [side 2]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[protein]-Npi-phospho-L-histidine + 2-O-alpha-mannosyl-D-glycerate [side 1]
-
715472
Escherichia coli
[protein]-L-histidine + 2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate [side 2]
-
-
-
?
Expression
Organism
Commentary
Expression
Escherichia coli
deletion of mngR results in the up-regulation of the gene mngA
up
Expression (protein specific)
Organism
Commentary
Expression
Escherichia coli
deletion of mngR results in the up-regulation of the gene mngA
up
Other publictions for EC 2.7.1.195
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715472
Sampaio
Phosphotransferase-mediated tr ...
Escherichia coli
J. Biol. Chem.
279
5537-5548
2004
-
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-