BRENDA - Enzyme Database show
show all sequences of 2.7.1.193

Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase

Rogers, M.J.; Ohgi, T.; Plumbridge, J.; Soell, D.; Gene 62, 197-207 (1988)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli IBPC463 cells
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68700
-
x * 68700, calculated from amino acid sequence
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[protein]-Npi-phospho-L-histidine + N-acetyl-D-glucosamine[side 1]
Escherichia coli
-
[protein]-L-histidine + N-acetyl-D-glucosamine 6-phosphate[side2]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P09323
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[protein]-Npi-phospho-L-histidine + N-acetyl-D-glucosamine[side 1]
-
736182
Escherichia coli
[protein]-L-histidine + N-acetyl-D-glucosamine 6-phosphate[side2]
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 68700, calculated from amino acid sequence
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli IBPC463 cells
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68700
-
x * 68700, calculated from amino acid sequence
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[protein]-Npi-phospho-L-histidine + N-acetyl-D-glucosamine[side 1]
Escherichia coli
-
[protein]-L-histidine + N-acetyl-D-glucosamine 6-phosphate[side2]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[protein]-Npi-phospho-L-histidine + N-acetyl-D-glucosamine[side 1]
-
736182
Escherichia coli
[protein]-L-histidine + N-acetyl-D-glucosamine 6-phosphate[side2]
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 68700, calculated from amino acid sequence
Escherichia coli
Other publictions for EC 2.7.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735545
Matano
Engineering of Corynebacterium ...
Corynebacterium glyciniphilum
Appl. Microbiol. Biotechnol.
98
5633-5643
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735492
Al Makishah
Dual substrate specificity of ...
Clostridium beijerinckii, Clostridium beijerinckii NCIMB8052
Appl. Environ. Microbiol.
79
6712-6718
2013
-
-
1
-
-
-
2
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712288
Plumbridge
An alternative route for recyc ...
Escherichia coli, Escherichia coli JW0662
J. Bacteriol.
191
5641-5647
2009
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
736897
Plumbridge
DNA binding sites for the Mlc ...
Escherichia coli
Nucleic Acids Res.
29
506-514
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
735790
Yamano
Cloning and sequencing of the ...
Vibrio cholerae, Vibrio cholerae non-O1 1148A
Biosci. Biotechnol. Biochem.
61
1349-1353
1997
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
736812
Vogler
Comparison of the sequences of ...
Escherichia coli, Klebsiella pneumoniae, Klebsiella pneumoniae KAY2051
Mol. Gen. Genet.
230
270-276
1991
-
-
-
-
-
-
-
-
2
-
-
3
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641861
Peri
Sequence of cloned enzyme IIN- ...
Escherichia coli
Biochemistry
27
6054-6061
1988
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736182
Rogers
Nucleotide sequences of the Es ...
Escherichia coli
Gene
62
197-207
1988
-
-
1
-
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-