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Literature summary for 2.7.1.191 extracted from
- Clostridioides difficile cd2775 encodes a unique mannosyl-1-phosphotransferase for polysaccharide II biosynthesis (2020), ACS Infect. Dis., 6, 680-686 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Clostridioides difficile |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.576 | - |
GDP-D-mannose | at pH 8.0 and 37°C | Clostridioides difficile |  |
| 0.934 | - |
Glcbeta1,3-GalNAc-PP-CPh | at pH 8.0 and 37°C | Clostridioides difficile |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridioides difficile | - |
- |
- |
| Clostridioides difficile 630 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Clostridioides difficile |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% (v/v) glycerol, 3 months, no loss of activity | Clostridioides difficile |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-2-azido-2-deoxy-D-glucose + Glcbeta1,3-GalNAc-PP-CPh | 61% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-2-azido-2-deoxy-D-glucose + Glcbeta1,3-GalNAc-PP-CPh | 61% activity compared to GDP-D-mannose | Clostridioides difficile 630 | ? | - |
? | |
| GDP-2-azido-2-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 86% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-2-azido-2-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 86% activity compared to GDP-D-mannose | Clostridioides difficile 630 | ? | - |
? | |
| GDP-4-azido-4-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 31% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-4-azido-4-deoxy-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 31% activity compared to GDP-D-mannose | Clostridioides difficile 630 | ? | - |
? | |
| GDP-D-glucopyranosylamine + Glcbeta1,3-GalNAc-PP-CPh | 67% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-D-glucose + Glcbeta1,3-GalNAc-PP-CPh | 66% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-D-mannobiosyl fluoride + Glcbeta1,3-GalNAc-PP-CPh | 88% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-D-mannopyranosylamine + Glcbeta1,3-GalNAc-PP-CPh | 64% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| GDP-D-mannose + Glcbeta1,3-GalNAc-PEG | - |
Clostridioides difficile | Man-alpha1-P-6-Glc-beta1,3-GalNAc-PEG + GMP | - |
? | |
| GDP-D-mannose + Glcbeta1,3-GalNAc-PP-CPh | 100% activity | Clostridioides difficile | Man-alpha1-P-6-Glc-beta1,3-GalNAc-PP-CPh + GMP | - |
? | |
| GDP-D-talose + Glcbeta1,3-GalNAc-PP-CPh | 48% activity compared to GDP-D-mannose | Clostridioides difficile | ? | - |
? | |
| additional information | the enzyme possesses a strict acceptor specificity toward Glc-beta1,3-GalNAc-diphospho-lipids | Clostridioides difficile | ? | - |
- |
|
| additional information | the enzyme shows no activity with GDP-Glc2dO, GDP-L-fucose, GDP-L-galactose, GTP, UDP-D-mannose, UDP-D-glucose, and CMPNeu5Ac | Clostridioides difficile | ? | - |
- |
|
| additional information | the enzyme possesses a strict acceptor specificity toward Glc-beta1,3-GalNAc-diphospho-lipids | Clostridioides difficile 630 | ? | - |
- |
|
| additional information | the enzyme shows no activity with GDP-Glc2dO, GDP-L-fucose, GDP-L-galactose, GTP, UDP-D-mannose, UDP-D-glucose, and CMPNeu5Ac | Clostridioides difficile 630 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant enzyme, SDS-PAGE | Clostridioides difficile |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CD2775 | - |
Clostridioides difficile |
| mannosyl-1-phosphotransferase | - |
Clostridioides difficile |
| ManPT | - |
Clostridioides difficile |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.248 | - |
GDP-D-mannose | at pH 8.0 and 37°C | Clostridioides difficile | |
| 2.738 | - |
Glcbeta1,3-GalNAc-PP-CPh | at pH 8.0 and 37°C | Clostridioides difficile |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is responsible for the formation of the Man-alpha1-P-6-Glc linkage in Clostridium difficile polysaccharide II | Clostridioides difficile |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.931 | - |
Glcbeta1,3-GalNAc-PP-CPh | at pH 8.0 and 37°C | Clostridioides difficile | |
| 3.903 | - |
GDP-D-mannose | at pH 8.0 and 37°C | Clostridioides difficile | |
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Release 2023.1 (January 2023)
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