Cloned (Comment) | Organism |
---|---|
gene aphSR2, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases and localized in one cluster of the Streptomyces rimosus strain ATCC 10970 genome, causing a 2fold increase in resistance to neomycin | Streptomyces rimosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + amikacin | Streptomyces rimosus | - |
GDP + amikacin 2''-phosphate | - |
? | |
GTP + amikacin | Streptomyces rimosus ATCC 10970 | - |
GDP + amikacin 2''-phosphate | - |
? | |
GTP + gentamicin | Streptomyces rimosus | - |
GDP + gentamicin 2''-phosphate | - |
? | |
GTP + gentamicin | Streptomyces rimosus ATCC 10970 | - |
GDP + gentamicin 2''-phosphate | - |
? | |
GTP + hygromycin | Streptomyces rimosus | - |
GDP + hygromycin 2''-phosphate | - |
? | |
GTP + hygromycin | Streptomyces rimosus ATCC 10970 | - |
GDP + hygromycin 2''-phosphate | - |
? | |
GTP + isepamycin | Streptomyces rimosus | - |
GDP + isepamycin 2''-phosphate | - |
? | |
GTP + kanamycin | Streptomyces rimosus | - |
GDP + kanamycin 2''-phosphate | - |
? | |
GTP + kanamycin | Streptomyces rimosus ATCC 10970 | - |
GDP + kanamycin 2''-phosphate | - |
? | |
GTP + neomycin | Streptomyces rimosus | - |
GDP + neomycin 2''-phosphate | - |
? | |
GTP + netilmycin | Streptomyces rimosus | - |
GDP + netilmycin 2''-phosphate | - |
? | |
GTP + sisomycin | Streptomyces rimosus | - |
GDP + sisomycin 2''-phosphate | - |
? | |
GTP + streptomycin | Streptomyces rimosus | - |
GDP + streptomycin 2''-phosphate | - |
? | |
GTP + tobramycin | Streptomyces rimosus | - |
GDP + tobramycin 2''-phosphate | - |
? | |
GTP + tobramycin | Streptomyces rimosus ATCC 10970 | - |
GDP + tobramycin 2''-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rimosus | - |
- |
- |
Streptomyces rimosus ATCC 10970 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + amikacin | - |
Streptomyces rimosus | GDP + amikacin 2''-phosphate | - |
? | |
GTP + amikacin | - |
Streptomyces rimosus ATCC 10970 | GDP + amikacin 2''-phosphate | - |
? | |
GTP + gentamicin | - |
Streptomyces rimosus | GDP + gentamicin 2''-phosphate | - |
? | |
GTP + gentamicin | - |
Streptomyces rimosus ATCC 10970 | GDP + gentamicin 2''-phosphate | - |
? | |
GTP + hygromycin | - |
Streptomyces rimosus | GDP + hygromycin 2''-phosphate | - |
? | |
GTP + hygromycin | - |
Streptomyces rimosus ATCC 10970 | GDP + hygromycin 2''-phosphate | - |
? | |
GTP + isepamycin | - |
Streptomyces rimosus | GDP + isepamycin 2''-phosphate | - |
? | |
GTP + kanamycin | - |
Streptomyces rimosus | GDP + kanamycin 2''-phosphate | - |
? | |
GTP + kanamycin | - |
Streptomyces rimosus ATCC 10970 | GDP + kanamycin 2''-phosphate | - |
? | |
GTP + neomycin | - |
Streptomyces rimosus | GDP + neomycin 2''-phosphate | - |
? | |
GTP + netilmycin | - |
Streptomyces rimosus | GDP + netilmycin 2''-phosphate | - |
? | |
GTP + sisomycin | - |
Streptomyces rimosus | GDP + sisomycin 2''-phosphate | - |
? | |
GTP + streptomycin | - |
Streptomyces rimosus | GDP + streptomycin 2''-phosphate | - |
? | |
GTP + tobramycin | - |
Streptomyces rimosus | GDP + tobramycin 2''-phosphate | - |
? | |
GTP + tobramycin | - |
Streptomyces rimosus ATCC 10970 | GDP + tobramycin 2''-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, recombinant enzyme, SDS-PAGE | Streptomyces rimosus |
Synonyms | Comment | Organism |
---|---|---|
aminoglycoside phosphotransferase | - |
Streptomyces rimosus |
AphSR2 | - |
Streptomyces rimosus |
General Information | Comment | Organism |
---|---|---|
evolution | Streptomyces rimosus ATCC 10970 contains 14 genes annotated as aminoglycoside phosphotransferases in its genome: aphSR1-aphSR14 | Streptomyces rimosus |
additional information | coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases, causes a 2fold increase in resistance to neomycin | Streptomyces rimosus |
physiological function | change in the level of resistance to aminoglycoside antibiotics upon combined expression of the aphSR2, pkSR1, and pkSR2 genes in Escherichia coli strain BL21(DE3), overview | Streptomyces rimosus |