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Literature summary for 2.7.1.190 extracted from

  • Rudakova, N.; Alekseeva, M.; Zakharevich, N.; Mavletova, D.; Danilenko, V.
    Aminoglycoside phosphotransferase AphSR2 from Streptomyces rimosus ATCC 10970 dependence of antibiotic Resistance on serine-threonine protein kinases PkSR1 and PkSR2 (2020), Russ. J. Genet., 56, 112-117 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene aphSR2, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases and localized in one cluster of the Streptomyces rimosus strain ATCC 10970 genome, causing a 2fold increase in resistance to neomycin Streptomyces rimosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + amikacin Streptomyces rimosus
-
GDP + amikacin 2''-phosphate
-
?
GTP + amikacin Streptomyces rimosus ATCC 10970
-
GDP + amikacin 2''-phosphate
-
?
GTP + gentamicin Streptomyces rimosus
-
GDP + gentamicin 2''-phosphate
-
?
GTP + gentamicin Streptomyces rimosus ATCC 10970
-
GDP + gentamicin 2''-phosphate
-
?
GTP + hygromycin Streptomyces rimosus
-
GDP + hygromycin 2''-phosphate
-
?
GTP + hygromycin Streptomyces rimosus ATCC 10970
-
GDP + hygromycin 2''-phosphate
-
?
GTP + isepamycin Streptomyces rimosus
-
GDP + isepamycin 2''-phosphate
-
?
GTP + kanamycin Streptomyces rimosus
-
GDP + kanamycin 2''-phosphate
-
?
GTP + kanamycin Streptomyces rimosus ATCC 10970
-
GDP + kanamycin 2''-phosphate
-
?
GTP + neomycin Streptomyces rimosus
-
GDP + neomycin 2''-phosphate
-
?
GTP + netilmycin Streptomyces rimosus
-
GDP + netilmycin 2''-phosphate
-
?
GTP + sisomycin Streptomyces rimosus
-
GDP + sisomycin 2''-phosphate
-
?
GTP + streptomycin Streptomyces rimosus
-
GDP + streptomycin 2''-phosphate
-
?
GTP + tobramycin Streptomyces rimosus
-
GDP + tobramycin 2''-phosphate
-
?
GTP + tobramycin Streptomyces rimosus ATCC 10970
-
GDP + tobramycin 2''-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces rimosus
-
-
-
Streptomyces rimosus ATCC 10970
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + amikacin
-
Streptomyces rimosus GDP + amikacin 2''-phosphate
-
?
GTP + amikacin
-
Streptomyces rimosus ATCC 10970 GDP + amikacin 2''-phosphate
-
?
GTP + gentamicin
-
Streptomyces rimosus GDP + gentamicin 2''-phosphate
-
?
GTP + gentamicin
-
Streptomyces rimosus ATCC 10970 GDP + gentamicin 2''-phosphate
-
?
GTP + hygromycin
-
Streptomyces rimosus GDP + hygromycin 2''-phosphate
-
?
GTP + hygromycin
-
Streptomyces rimosus ATCC 10970 GDP + hygromycin 2''-phosphate
-
?
GTP + isepamycin
-
Streptomyces rimosus GDP + isepamycin 2''-phosphate
-
?
GTP + kanamycin
-
Streptomyces rimosus GDP + kanamycin 2''-phosphate
-
?
GTP + kanamycin
-
Streptomyces rimosus ATCC 10970 GDP + kanamycin 2''-phosphate
-
?
GTP + neomycin
-
Streptomyces rimosus GDP + neomycin 2''-phosphate
-
?
GTP + netilmycin
-
Streptomyces rimosus GDP + netilmycin 2''-phosphate
-
?
GTP + sisomycin
-
Streptomyces rimosus GDP + sisomycin 2''-phosphate
-
?
GTP + streptomycin
-
Streptomyces rimosus GDP + streptomycin 2''-phosphate
-
?
GTP + tobramycin
-
Streptomyces rimosus GDP + tobramycin 2''-phosphate
-
?
GTP + tobramycin
-
Streptomyces rimosus ATCC 10970 GDP + tobramycin 2''-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 41000, recombinant enzyme, SDS-PAGE Streptomyces rimosus

Synonyms

Synonyms Comment Organism
aminoglycoside phosphotransferase
-
Streptomyces rimosus
AphSR2
-
Streptomyces rimosus

General Information

General Information Comment Organism
evolution Streptomyces rimosus ATCC 10970 contains 14 genes annotated as aminoglycoside phosphotransferases in its genome: aphSR1-aphSR14 Streptomyces rimosus
additional information coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases, causes a 2fold increase in resistance to neomycin Streptomyces rimosus
physiological function change in the level of resistance to aminoglycoside antibiotics upon combined expression of the aphSR2, pkSR1, and pkSR2 genes in Escherichia coli strain BL21(DE3), overview Streptomyces rimosus