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Literature summary for 2.7.1.190 extracted from
- Revisiting the nucleotide and aminoglycoside substrate specificity of the bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia enzyme (2012), J. Biol. Chem., 287, 43262-43269.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butirosin A/B | - |
Staphylococcus aureus |  |
| lividomycin A | - |
Staphylococcus aureus | |
| neomycin B | - |
Staphylococcus aureus | |
| paromomycin | - |
Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
dibekacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.0008 | - |
sisomicin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.0008 | - |
arbekacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.0041 | - |
netilmicin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.006 | - |
gentamicin C | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.033 | - |
amikacin | pH 7.0, 22°C | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Staphylococcus aureus | GTP is the exclusive phosphate donor at intracellular nucleotide levels | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | P0A0C1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + amikacin | - |
Staphylococcus aureus | GDP + amikacin 2''-phosphate | - |
? | |
| GTP + arbekacin | - |
Staphylococcus aureus | GDP + arbekacin 2''-phosphate | - |
? | |
| GTP + dibekacin | - |
Staphylococcus aureus | GDP + dibekacin 2''-phosphate | - |
? | |
| GTP + gentamicin C | - |
Staphylococcus aureus | GDP + gentamicin C 2''-phosphate | - |
? | |
| GTP + kanamycin A | - |
Staphylococcus aureus | GDP + kanamycin A 2''-phosphate | - |
? | |
| GTP + kanamycin B | - |
Staphylococcus aureus | GDP + kanamycin B 2''-phosphate | - |
? | |
| GTP + netilmicin | - |
Staphylococcus aureus | GDP + netilmicin 2''-phosphate | - |
? | |
| GTP + sisomicin | - |
Staphylococcus aureus | GDP + sisomicin 2''-phosphate | - |
? | |
| GTP + tobramycin | - |
Staphylococcus aureus | GDP + tobramycin 2''-phosphate | - |
? | |
| additional information | GTP is the exclusive phosphate donor at intracellular nucleotide levels | Staphylococcus aureus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia | - |
Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
tobramycin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.12 | - |
kanamycin B | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.15 | - |
dibekacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.57 | - |
kanamycin A | pH 7.0, 22°C | Staphylococcus aureus | |
| 2.3 | - |
arbekacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 3 | - |
gentamicin C | pH 7.0, 22°C | Staphylococcus aureus | |
| 3.7 | - |
amikacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 6.5 | - |
netilmicin | pH 7.0, 22°C | Staphylococcus aureus | |
| 8.1 | - |
sisomicin | pH 7.0, 22°C | Staphylococcus aureus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00026 | - |
neomycin B | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.0053 | - |
paromomycin | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.011 | - |
lividomycin A | pH 7.0, 22°C | Staphylococcus aureus | |
| 0.14 | - |
butirosin A/B | pH 7.0, 22°C | Staphylococcus aureus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme phosphorylates 4,6-disubstituted aminoglycosides with high efficiency. Despite this proficiency, no resistance is conferred to some of these antibiotics by the enzyme in vivo. Phosphorylation of 4,5-disubstituted and atypical aminoglycosides are negligible and these antibiotics are not substrates. Instead, these aminoglycosides tend to stimulate an intrinsic GTPase activity of the enzyme | Staphylococcus aureus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 110 | - |
amikacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 500 | - |
dibekacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 2900 | - |
arbekacin | pH 7.0, 22°C | Staphylococcus aureus | |
| 5000 | - |
gentamicin C | pH 7.0, 22°C | Staphylococcus aureus | |
| 10000 | - |
sisomicin | pH 7.0, 22°C | Staphylococcus aureus | |
| 16000 | - |
netilmicin | pH 7.0, 22°C | Staphylococcus aureus | |
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