Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.19 extracted from

  • Runquist, J.A.; Rios, S.E.; Vinarov, D.A.; Miziorko, H.M.
    Functional evaluation of serine/threonine residues in the P-loop of Rhodobacter sphaeroides phosphoribulokinase (2001), Biochemistry, 400, 14530-14537.
    View publication on PubMed
Search for more literature for 2.7.1.19

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
S14A decreased Vmax by 40fold Cereibacter sphaeroides
S19A ability to form complexes with Mg-ATP2-, decreased Vmax, increased Km for D-ribulose 5-phosphate Cereibacter sphaeroides
T18A ability to form complexes with Mg-ATP2-, increased Km for D-ribulose 5-phosphate, decreased Vmax Cereibacter sphaeroides
T20A ability to form complexes with Mg-ATP2- Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
6-phospho-D-gluconate
-
 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.096
-
 D-ribulose 5-phosphate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
0.586
-
 D-ribulose 5-phosphate S14A mutant, pH 8, 30°C Cereibacter sphaeroides
0.662
-
 D-ribulose 5-phosphate T20A mutant, pH 8, 30°C Cereibacter sphaeroides
3.16
-
 D-ribulose 5-phosphate S19A mutant, pH 8, 30°C Cereibacter sphaeroides
4.5
-
 D-ribulose 5-phosphate T18A mutant, pH 8, 30°C Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 native enzyme Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + ATP
-
 Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

Subunits Comment Organism
octamer 8 *32000, SDS-PAGE Cereibacter sphaeroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 6-phospho-D-gluconate T18A mutant, pH 8, 30°C Cereibacter sphaeroides
0.22
-
 6-phospho-D-gluconate wild-type enzyme and S19A mutant, pH 8, 30°C Cereibacter sphaeroides